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Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness.
Emerging Infectious Diseases ( IF 11.8 ) Pub Date : 2020-02-01 , DOI: 10.3201/eid2602.191351
Heather P. McLaughlin , Julia V. Bugrysheva , Andrew B. Conley , Christopher A. Gulvik , Blake Cherney , Cari B. Kolton , Chung K. Marston , Elke Saile , Erin Swaney , David Lonsway , Amy S. Gargis , Thiphasone Kongphet-Tran , Christine Lascols , Pierre Michel , Julie Villanueva , Alex R. Hoffmaster , Jay E. Gee , David Sue

Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response.

中文翻译:

快速纳米孔全基因组测序,用于炭疽应急准备。

人炭疽病例需要快速反应。我们在收到细菌后数小时即可从人类炭疽分离物中的高含量实验室中完成了炭疽杆菌纳米孔全基因组测序。从头组装的基因组没有显示出已知的抗药性耐药基因或引入的质粒的证据。当天的基因组表征可增强公共卫生应急响应。
更新日期:2020-01-21
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