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Electrochemical sensing of hydrogen peroxide using a glassy carbon electrode modified with multiwalled carbon nanotubes and zein nanoparticle composites: application to HepG2 cancer cell detection
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-01-08 , DOI: 10.1007/s00604-019-4064-7
Hamed Tavakkoli 1 , Morteza Akhond 1 , Gholam Abbas Ghorbankhani 2 , Ghodratollah Absalan 1
Affiliation  

A nanobiocomposite was prepared from multiwalled carbon nanotubes and zein nanoparticles. It was dispersed in water/ethanol and drop cast onto a glassy carbon electrode. The modified electrode can be used for electroreduction of H 2 O 2 (typically at a working potential of −0.71 V vs. Ag/AgCl). The electrochemical properties of the electrode were investigated by cyclic voltammetry, linear sweep voltammetry, chronoamperometry and electrochemical impedance spectroscopy. Response to H 2 O 2 is linear in the 0.049 to 22 μM concentration range, and the detection limit is 35 nM at pH 7.0. The sensor was successfully utilized for the measurement of H 2 O 2 in a synthetic urine sample, and for monitoring the release of H 2 O 2 from human dermal fibroblasts and human hepatocellular carcinoma cells. Graphical abstract Schematic representation of a novel metal- and enzyme-free electrochemical nanosensor. A glassy carbon electrode was modified with a nanocomposite prepared from multiwalled carbon nanotubes and zein nanoparticles. It was applied to the identification of liver cancer cells via sensing of H2O2 and has a very low detection limit.

中文翻译:

使用多壁碳纳米管和玉米醇溶蛋白纳米颗粒复合材料修饰的玻璃碳电极对过氧化氢的电化学传感:应用于 HepG2 癌细胞检测

从多壁碳纳米管和玉米醇溶蛋白纳米颗粒制备纳米生物复合材料。将其分散在水/乙醇中并滴铸到玻璃碳电极上。修改后的电极可用于 H 2 O 2 的电还原(通常在 -0.71 V 相对于 Ag/AgCl 的工作电位下)。通过循环伏安法、线性扫描伏安法、计时电流法和电化学阻抗谱研究了电极的电化学性能。对 H 2 O 2 的响应在 0.049 至 22 μM 浓度范围内呈线性,在 pH 7.0 时检测限为 35 nM。该传感器已成功用于测量合成尿样中的 H 2 O 2 ,​​并用于监测人皮肤成纤维细胞和人肝细胞癌细胞中 H 2 O 2 的释放。图形摘要 新型无金属和无酶电化学纳米传感器的示意图。用由多壁碳纳米管和玉米醇溶蛋白纳米颗粒制备的纳米复合材料对玻碳电极进行改性。它通过感知H2O2应用于肝癌细胞的鉴定,具有极低的检出限。
更新日期:2020-01-08
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