A previously isolated a bacteriophage, vB_EcoS_AKFV33 of T5virus, demonstrated great potential in biocontrol of Shiga toxigenic Escherichia coli (STEC) O157. This study further evaluated its potential as a biocontrol agent in broth culture against other important non-O157 serogroups of STEC and Salmonella. AKFV33 was capable of lysing isolates of STEC serogroups O26 (n = 1), O145 (n = 1) and Salmonella enterica serovars (n = 6). In a broth culture microplate system, efficacy of AKFV33 for killing STEC O26:H11, O145:NM and Salmonella was improved (P < 0.05) at a lower multiplicity of infection and sampling time (6-10 h), when STEC O157:H7 was also included in the culture. This phage was able to simultaneously reduce numbers of STEC and Salmonella in mixtures with enhanced activity (P < 0.05) against O157:H7 and O26:H11, offering great promise for control of multiple zoonotic pathogens at both pre and post-harvest.

中文翻译：

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噬菌体T5病毒对志贺氏菌产毒大肠杆菌和沙门氏菌菌株在单个和混合培养物中的生长的影响。

先前分离出的T5病毒噬菌体vB_EcoS_AKFV33在志贺产毒原性大肠杆菌（STEC）O157的生物防治中显示出巨大潜力。这项研究进一步评估了其在肉汤培养中针对STEC和沙门氏菌其他重要的非O157血清群的潜力。AKFV33能够裂解STEC血清群O26（n = 1），O145（n = 1）和肠炎沙门氏菌（n = 6）的分离株。在肉汤培养微孔板系统中，当STEC O157：H7降低感染和取样时间（6-10小时）时，AKFV33杀死STEC O26：H11，O145：NM和沙门氏菌的功效得到改善（P <0.05）。也包括在文化中。该噬菌体能够同时减少混合物中STEC和沙门氏菌的数量，并且具有增强的针对O157：H7和O26：H11的活性（P <0.05），