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Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles.
Journal of Nanobiotechnology ( IF 10.2 ) Pub Date : 2020-01-07 , DOI: 10.1186/s12951-019-0569-9
Narsingh R Nirala 1 , Yifat Harel 2 , Jean-Paul Lellouche 2 , Giorgi Shtenberg 1
Affiliation  

BACKGROUND Haptoglobin is an acute-phase protein used as predicting diagnostic biomarker both in humans (i.e., diabetes, ovarian cancer, some neurological and cardiovascular disorders) and in animals (e.g., bovine mastitis). The latter is a frequent disease of dairy industry with staggering economical losses upon decreased milk production and increased health care costs. Early stage diagnosis of the associated diseases or inflammation onset is almost impossible by conventional analytical manners. RESULTS The present study demonstrates a simple, rapid, and cost-effective label-free chemiluminescence bioassay based on magnetite nanoparticles (MNPs) for sensitive detection of haptoglobin by employing the specific interaction of hemoglobin-modified MNPs. The resulting haptoglobin-hemoglobin complex inhibits the peroxidase-like activity of luminol/H2O2-hemoglobin-MNPs sensing scheme and reduces the chemiluminescence intensities correspondingly to the innate haptoglobin concentrations. Quantitative detection of bovine haptoglobin was obtained within the range of 1 pg mL-1 to 1 µg mL-1, while presenting 0.89 pg mL-1 limit of detection. Moreover, the influence of causative pathogenic bacteria (i.e., Streptococcus dysgalactiae and Escherichia coli) and somatic cell counts (depicting healthy, sub-clinical and clinical mastitis) on the emitted chemiluminescence radiation were established. The presented bioassay quantitative performances correspond with a standardized assay kit in differentiating dissimilar milk qualities. CONCLUSIONS Overall, the main advantage of the presented sensing concept is the ability to detect haptoglobin, at clinically relevant concentrations within real milk samples for early bio-diagnostic detection of mastitis and hence adjusting the precise treatment, potentially initiating a positive influence on animals' individual health and hence on dairy farms economy.

中文翻译:

基于磁铁矿纳米颗粒化学发光的超灵敏触觉珠蛋白生物标志物检测。

背景技术肝珠蛋白是一种急性期蛋白,在人类(即糖尿病,卵巢癌,某些神经系统疾病和心血管疾病)和动物(例如牛乳腺炎)中均被用作预测诊断性生物标志物。后者是乳制品行业的常见病,由于牛奶产量减少和医疗保健成本增加,经济损失惊人。通过常规分析方法几乎不可能对相关疾病或炎症发作进行早期诊断。结果本研究证明了一种简单,快速且经济高效的无标记化学发光生物测定方法,该方法基于磁铁矿纳米颗粒(MNP),可通过血红蛋白修饰的MNP的特异性相互作用来灵敏地检测触觉珠蛋白。所得的触珠蛋白-血红蛋白复合物抑制了鲁米诺/ H2O2-血红蛋白-MNPs传感方案的过氧化物酶样活性,并降低了与先天触珠蛋白浓度相对应的化学发光强度。牛触珠蛋白的定量检测范围为1 pg mL-1至1 µg mL-1,而检测限为0.89 pg mL-1。此外,确定了致病性病原菌(即链球菌dysgalactiae和大肠杆菌)和体细胞计数(描述健康的,亚临床的和临床的乳腺炎)对发出的化学发光辐射的影响。提出的生物测定定量性能与标准化的测定试剂盒在区分不同牛奶质量方面相对应。结论总体而言,
更新日期:2020-01-07
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