The antimicrobial capability and recyclability of two conjugates that combines the versatility of iron oxide magnetic nanoparticles (MNPs) with the high photosensitizing proficiency of boron-dipyrromethene (BODIPY) dyes are assessed. By a relatively simple synthetic pathway, two conjugates were obtained. The first one, MNP-B1, contains a highly fluorescent dye for bioimaging and suitable inactivating properties. The other one, MNP-B2, is optimized to improve the production of cytotoxic reactive oxygen species (ROS) by incorporating heavy atoms in the BODIPY core. In vitro experiments in bacterial cell suspensions and at the single bacterium level reveal that both conjugates can inactivate either Gram-positive (methicillin-resistant Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. By means of fluorescence microscopy, not only cellular uptake of the conjugates but also recyclability and sustained performance over the cycles of photodynamic inactivation (PDI) are demonstrated. This is the first time that MNPs functionalized with BODIPY dyes are utilized to obtain fluorescent images of bacterial cells and photoinactivate pathogens.

中文翻译：

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用于生物成像和抗菌治疗应用的具有 BODIPY 的功能化磁性纳米粒子

评估了将氧化铁磁性纳米粒子 (MNP) 的多功能性与硼-二吡咯亚甲基 (BODIPY) 染料的高光敏能力相结合的两种结合物的抗菌能力和可回收性。通过相对简单的合成途径，获得了两种缀合物。第一个，MNP-B1，包含一种用于生物成像的高荧光染料和合适的灭活特性。另一种是 MNP-B2，经过优化，通过在 BODIPY 核心中加入重原子来改善细胞毒性活性氧 (ROS) 的产生。细菌细胞悬液和单一细菌水平的体外实验表明，两种结合物均可灭活革兰氏阳性（耐甲氧西林金黄色葡萄球菌）和革兰氏阴性（大肠杆菌）） 细菌。通过荧光显微镜，不仅证明了结合物的细胞摄取，而且证明了光动力灭活 (PDI) 循环中的可回收性和持续性能。这是第一次使用 BODIPY 染料功能化的 MNPs 来获得细菌细胞的荧光图像和光灭活病原体。