Water-in-oil (w/o) Pickering emulsions have received considerable attention in biphasic enzymatic catalysis for their advantages of good stability, large interfacial area, and ease of product separation. However, enzymes are commonly encapsulated in the interior of aqueous droplets, which inevitably increases the diffusional resistance to catalysis. Alternatively, enzymes are immobilized or trapped into Pickering stabilizers. Often, however, these approaches suffer from leaching and a decrease of enzyme activity during the chemical treatments. We report here a new Pickering interfacial biocatalysis platform with efficient enzyme encapsulation, binary particle composition, and high catalytic performance. Our approach is based on w/o Pickering emulsions stabilized by binary particles consisting of hard silica and soft, pH-responsive microgel particles. We demonstrate that pH-responsive microgels can simultaneously stabilize a w/o Pickering emulsion, encapsulate enzymes, and catalyze reactions at the water/oil interface. In addition, we show that the coordination with rigid silica nanoparticles as additional stabilizers markedly improves the emulsion structure and will provide a new avenue for the preparation of w/o Pickering emulsion and concept of biphasic catalysis.

中文翻译：

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由具有相反特性和功能的二元颗粒稳定的逆挑料乳液，用于界面生物催化。

油包水（w / o）Pickering乳液因其良好的稳定性，大的界面面积和易于分离的优点而在双相酶催化中受到了广泛的关注。然而，酶通常被包封在水滴的内部，这不可避免地增加了对催化的扩散阻力。或者，将酶固定或捕获到Pickering稳定剂中。但是，这些方法经常在化学处理过程中遭受浸提和酶活性降低。我们在这里报告了一个新的Pickering界面生物催化平台，该平台具有高效的酶包封，二元颗粒组成和高催化性能。我们的方法基于不带Pickering乳液的乳液，该乳液由由硬二氧化硅和软二氧化硅组成的二元颗粒稳定，pH响应微凝胶颗粒。我们证明，pH响应微凝胶可以同时稳定w / o Pickering乳液，包封酶并催化水/油界面处的反应。另外，我们表明与刚性二氧化硅纳米颗粒作为另外的稳定剂的配位显着改善了乳液结构，并将为制备不含水Pickering乳液和双相催化的概念提供新途径。