Current techniques for intracellular electrical interrogation are limited by substrate-bound devices, technically demanding methods, or insufficient spatial resolution. In this work, we use freestanding silicon nanowires to achieve photoelectric stimulation in myofibroblasts with subcellular resolution. We demonstrate that myofibroblasts spontaneously internalize silicon nanowires and subsequently remain viable and capable of mitosis. We then show that stimulation of silicon nanowires at separate intracellular locations results in local calcium fluxes in subcellular regions. Moreover, nanowire-myofibroblast hybrids electrically couple with cardiomyocytes in coculture, and photostimulation of the nanowires increases the spontaneous activation rate in coupled cardiomyocytes. Finally, we demonstrate that this methodology can be extended to the interrogation of signaling in neuron-glia interactions using nanowire-containing oligodendrocytes.

中文翻译：

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硅纳米线用于亚细胞分辨率的细胞内光学询问。

当前用于细胞内电询问的技术受到基质结合的装置，技术要求高的方法或空间分辨率不足的限制。在这项工作中，我们使用独立的硅纳米线以亚细胞分辨率在成肌纤维细胞中实现光电刺激。我们证明成肌纤维细胞自发内在化硅纳米线，并随后保持活力和有丝分裂的能力。然后，我们表明在单独的细胞内位置刺激硅纳米线会导致亚细胞区域的局部钙通量。此外，在共培养中，纳米线-肌成纤维细胞杂合体与心肌细胞电耦合，并且纳米线的光刺激增加了耦合的心肌细胞的自发活化率。最后，