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Biotransformation of 2,4,6-Trinitrotoluene by Pseudomonas sp. TNT3 isolated from Deception Island, Antarctica.
Environmental Pollution ( IF 8.9 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.envpol.2020.113922
Ma Ángeles Cabrera 1 , Sebastián L Márquez 1 , Carolina P Quezada 1 , Manuel I Osorio 1 , Eduardo Castro-Nallar 1 , Fernando D González-Nilo 1 , José M Pérez-Donoso 1
Affiliation  

2,4,6-Trinitrotoluene (TNT) is a nitroaromatic explosive, highly toxic and mutagenic for organisms. In this study, we report for the first time the screening and isolation of TNT-degrading bacteria from Antarctic environmental samples with potential use as bioremediation agents. Ten TNT-degrading bacterial strains were isolated from Deception Island. Among them, Pseudomonas sp. TNT3 was selected as the best candidate since it showed the highest tolerance, growth, and TNT biotransformation capabilities. Our results showed that TNT biotransformation involves the reduction of the nitro groups. Additionally, Pseudomonas sp. TNT3 was capable of transforming 100 mg/L TNT within 48 h at 28 °C, showing higher biotransformation capability than Pseudomonas putida KT2440, a known TNT-degrading bacterium. Functional annotation of Pseudomonas sp. TNT3 genome revealed a versatile set of molecular functions involved in xenobiotic degradation pathways. Two putative xenobiotic reductases (XenA_TNT3 and XenB_TNT3) were identified by means of homology searches and phylogenetic relationships. These enzymes were also characterized at molecular level using homology modelling and molecular dynamics simulations. Both enzymes share different levels of sequence similarity with other previously described TNT-degrading enzymes and with their closest potential homologues in databases.

中文翻译:

假单胞菌属物种对2,4,6-三硝基甲苯的生物转化。从南极洲欺骗岛分离的TNT3。

2,4,6-三硝基甲苯(TNT)是一种硝基芳香族炸药,对生物具有高毒性和致突变性。在这项研究中,我们首次报告了从南极环境样品中筛选和分离TNT降解细菌的潜力,这些细菌有可能用作生物修复剂。从欺骗岛分离出十种降解TNT的细菌菌株。其中,假单胞菌属。TNT3被选为最佳候选者,因为它显示出最高的耐受性,生长和TNT生物转化能力。我们的结果表明,TNT的生物转化涉及硝基的还原。此外,假单胞菌属。TNT3能够在28°C下48小时内转化100 mg / L TNT,显示出比恶臭假单胞菌KT2440(一种已知的TNT降解细菌)更高的生物转化能力。假单胞菌属的功能注释。TNT3基因组揭示了一组与异源生物降解途径有关的分子功能。通过同源搜索和系统发育关系鉴定了两种推定的异源生物还原酶(XenA_TNT3和XenB_TNT3)。还使用同源性建模和分子动力学模拟在分子水平上表征了这些酶。两种酶与其他先前描述的TNT降解酶以及它们在数据库中最接近的潜在同源物具有不同水平的序列相似性。还使用同源性建模和分子动力学模拟在分子水平上表征了这些酶。两种酶与其他先前描述的TNT降解酶以及它们在数据库中最接近的潜在同源物具有不同水平的序列相似性。还使用同源性建模和分子动力学模拟在分子水平上表征了这些酶。两种酶与其他先前描述的TNT降解酶以及它们在数据库中最接近的潜在同源物具有不同水平的序列相似性。
更新日期:2020-01-07
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