An electrochemiluminescence and photothermal immunosensor based on a dual-modality integrated probe was proposed for sensitive and reliable detection of lipolysis stimulated lipoprotein receptor (LSR), a new biomarker of ovarian cancer. Black phosphorous quantum dots (BPQDs) possess fascinating electrochemical property and unique photothermal effect, which could not only enhance ECL signal of N-(4-aminobutyl)-N-ethylisoluminol (ABEI) through accelerating dissolved O2 evolution but also realize temperature signal output by converting laser energy into heat. Furthermore, NiFe2O4 nanotubes (NiFe2O4 NTs) have large specific surface area and favorable adsorption ability, which could increase the immobilized amount of ABEI and BPQDs, further strengthening ECL and temperature signal. As a result, a dual-mode immunosensor was constructed and realized ECL and temperature dual signal to detect LSR, making the results more reliable. This work provided a new thought for the development of sensitive and accurate sensors and was expected to employ for determination of other biomarkers.

中文翻译：

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基于黑色磷和NiFe2O4 NTs的双峰探针用于电化学发光和光热检测卵巢癌标志物。

提出了一种基于双模态整合探针的电化学发光和光热免疫传感器，用于灵敏和可靠地检测脂解刺激的脂蛋白受体（LSR），这是卵巢癌的一种新生物标记。黑磷量子点（BPQDs）具有令人着迷的电化学性能和独特的光热效应，不仅可以通过加速溶解的O2释放来增强N-（4-氨基丁基）-N-乙基异鲁米诺（ABEI）的ECL信号，而且还可以实现将激光能量转化为热量。此外，NiFe2O4纳米管（NiFe2O4 NTs）具有较大的比表面积和良好的吸附能力，可以增加ABEI和BPQDs的固定量，从而进一步增强ECL和温度信号。结果是，构建了双模式免疫传感器，实现了ECL和温度双信号检测LSR，结果更加可靠。这项工作为开发灵敏和准确的传感器提供了新思路，并有望用于确定其他生物标志物。