To promote the detection sensitivity of neutral analytes, a sensitive two-step stacking strategy by coupling field-enhanced sample injection and micelle to cyclodextrin stacking in reverse migrating micelles MEKC has been established by selecting jujuboside A and B as models. The stacking mechanism, affecting factors as well as analytical performances of the proposed method were investigated. Compared with typical injection, the sensitivity enhancement factors of this method were up to 140- and 152-fold for jujuboside A and B, respectively. The enhancements were 8-10 times better than only single stacking strategy by MCDS. The LODs were 0.2-0.3 μg mL-1, interday and intraday%RSD were both lower than 4.3%, and a good linearity (r>0.999) was obtained. Feasibility in real samples analysis was evaluated by using Semen Ziziphi Spinosae dispensing granule and rat's urine. The results suggested that the strategy was simple, reliable, sensitive and promising for the analysis of neutral analytes in complex sample matrix.

中文翻译：

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通过将场增强的样品注入和胶束与环糊精的偶联耦合进行灵敏的两步法测定中性分析物。

为了提高中性分析物的检测灵敏度，通过选择大枣糖苷A和B作为模型，建立了通过将场增强的样品注射和胶束与环糊精堆叠的反向迁移胶束MEKC耦合的灵敏两步堆叠策略。研究了该方法的叠加机理，影响因素以及分析性能。与典型的注射剂相比，该方法对大枣糖苷A和B的敏感性增强因子分别高达140倍和152倍。增强效果比仅MCDS的单个堆叠策略好8-10倍。LOD为0.2-0.3μgmL-1，日间和日间的％RSD均低于4.3％，并获得了良好的线性度（r> 0.999）。通过使用Semen Ziziphi Spinosae分配颗粒和大鼠尿液评估实际样品分析的可行性。结果表明，该策略简单，可靠，灵敏，对分析复杂样品基质中的中性分析物很有前途。