Ion mobility spectrometry was utilized to corroborate the identity of streptorubin B (2) as the natural product produced by Streptomyces coelicolor. Natural product 2 was initially assigned as butylcycloheptylprodigiosin (3), and only relatively recently was this assignment clarified. We present additional evidence of this assignment by comparing collisional cross sections (Ω) of synthetic standards of 2, 3, and metacycloprodigiosin (4) to the cyclic prodiginine produced by S. coelicolor. Calculated theoretical Ω values demonstrate that cyclic prodiginines could be identified without standards. This work highlights ion mobility as an efficient tool for the dereplication of natural products.

中文翻译：

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离子淌度质谱作为鉴定天蓝色链霉菌M145中链霉菌素B的有效工具。

离子迁移谱用于证实链霉菌素B（2）是天蓝色链霉菌产生的天然产物的身份。最初将天然产物2命名为丁基环庚基prodigiosin（3），直到最近才明确这种分配。通过比较合成标准品2，3和metacycloprodigiosin（4）的碰撞横截面（Ω）与co.color产生的环状prodiginine，我们提供了这种分配的其他证据。计算得出的理论Ω值表明，可以在没有标准的情况下识别循环蛋白质。这项工作突出了离子迁移率，将其作为天然产物去复制的有效工具。