The fluctuation of cholesterol in plasma membrane at single cells during testosterone treatment was evaluated using luminol electrochemiluminescence at the first time. Testosterone over the human physiological concentration induced more membrane cholesterol that was associated with upregulated cholesterol synthesis inside the cells. However, the evaluation at single cell level revealed some individual cells with similar membrane cholesterol, while the other individual cells with more membrane cholesterol after the treatment. The observation of non‐responsive cells to testosterone treatment exhibited the heterogeneous cellular function during this process, which should provide more information to understand the side effects of testosterone replacement therapy.

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睾丸激素治疗过程中血浆膜胆固醇的单细胞电化学发光分析

首次使用鲁米诺电化学发光法评估睾丸激素治疗期间单细胞质膜胆固醇的波动。超过人类生理浓度的睾丸激素诱导更多的膜胆固醇，这与细胞内部胆固醇合成上调有关。但是，单细胞水平的评估显示，处理后某些单个细胞的膜胆固醇相似，而其他单个细胞的膜胆固醇更高。观察到对睾丸激素治疗无反应的细胞在此过程中表现出异质性细胞功能，这应提供更多信息，以了解睾丸激素替代疗法的副作用。