Abstract MicroRNA is crucial for post-transcription gene regulation and is a new type of biomarker for many diseases. Herein, we synthesized a novel bisferrocene biomarker, which was placed at both ends of hairpin DNA, generating a probe of high-efficiency amplification signal. Our method entailed the integration of the catalytic hairpin assembly (CHA) and bisferrocenes into a double-amplified signal electrochemical miRNA biosensor. The biosensor achieved ultra-sensitive detection of miRNA-21 with a minimum detection limit of 0.1 f. and linear range of 0.2 f. ~ 0.2 nM. This study provides an effective detection way for miRNA, and opens new possibilities for future clinical diagnosis and treatment of diseases.

中文翻译：

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基于催化发夹组装和双二茂铁标记的双信号放大电化学MicroRNA生物传感器

摘要 MicroRNA 对转录后基因调控至关重要，是多种疾病的新型生物标志物。在此，我们合成了一种新型双二茂铁生物标志物，将其置于发夹 DNA 的两端，产生高效放大信号的探针。我们的方法需要将催化发夹组件 (CHA) 和双二茂铁集成到双放大信号电化学 miRNA 生物传感器中。该生物传感器实现了对 miRNA-21 的超灵敏检测，最低检测限为 0.1 f。和 0.2 f 的线性范围。~ 0.2 纳米。该研究为miRNA提供了一种有效的检测方法，为未来临床疾病的诊断和治疗开辟了新的可能性。