Pluripotent stem cells (PSCs) offer a promising tool for regenerative medicine. The clinical application of PSCs inevitably requires a large-scale culture in a highly defined environment. The present study aimed to devise defined coating materials for the efficient adhesion and proliferation of human PSCs (hPSCs). We tested the activity of seven fibronectin-derived peptides and three laminin-derived peptides for the attachment and proliferation of hPSCs through their immobilization on the bottom of culture dishes by creating a fusion protein with the mussel adhesion protein. Among the extracellular matrix (ECM) mimetics tested, one fibronectin-derived peptide, PHSRN-GRGDSP, significantly promoted adhesion, enhanced alkaline phosphatase activity, and increased pluripotency-related gene expression in hPSCs compared to Matrigel. Furthermore, co-immobilization of a particular canofin peptide derived from fibroblast growth factor 2 increased pluripotency marker expression, which may offer the possibility of culture without growth factor supplementation. Our findings afford a novel defined condition for the efficient culture of hPSCs and may be utilized in future clinical applications.

多能干细胞（PSC）为再生医学提供了一种有前途的工具。PSC的临床应用不可避免地需要在高度限定的环境中进行大规模培养。本研究旨在设计用于人类PSC（hPSC）的有效粘附和增殖的定义涂层材料。我们通过创建与贻贝粘附蛋白的融合蛋白，通过将hPSC固定在培养皿底部，测试了7种纤连蛋白衍生肽和3种层粘连蛋白衍生肽对hPSC的附着和增殖的活性。与Matrigel相比，在测试的细胞外基质（ECM）模拟物中，一种纤连蛋白衍生肽PHSRN-GRGDSP显着促进了hPSC中的粘附，增强了碱性磷酸酶活性并增加了多能性相关基因的表达。此外，共同固定源自成纤维细胞生长因子2的特定canofin肽可提高多能性标记物的表达，这可能提供不补充生长因子的培养可能性。我们的发现为hPSC的有效培养提供了一种新的明确条件，并可用于未来的临床应用中。