Herbal medicine has attracted great interest throughout the world. However, separation and characterization of components in herbs is still a challenge. Advanced techniques involved in this task, mainly including hydrophilic interaction liquid chromatography, supercritical fluid chromatography, two-dimensional chromatography and preparative chromatography, have showing an ongoing development in recent years. In this review, in addition to summarizing their progress and applications in herbal medicine in the last five years, their combination with mass spectrometry, activity screening or other techniques were introduced in detail.

中文翻译：

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用草药对复杂样品进行化学分离和表征

草药引起了全世界的极大兴趣。然而，草药中成分的分离和表征仍然是一个挑战。这项任务涉及的先进技术，主要包括亲水作用液相色谱法，超临界流体色谱法，二维色谱法和制备色谱法，近年来已显示出不断发展的技术。在这篇综述中，除了总结它们在过去五年中在草药中的进展和应用之外，还详细介绍了它们与质谱，活性筛选或其他技术的结合。