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Sleep Loss Immediately After Fear Memory Reactivation Attenuates Fear Memory Reconsolidation.
Neuroscience ( IF 3.3 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.neuroscience.2019.12.024
Rishi Sharma 1 , Pradeep Sahota 1 , Mahesh M Thakkar 1
Affiliation  

Permanently stored memories become labile through a process called reactivation. Once reactivated, these memories need reconsolidation to become permanent. Sleep is critical for memory consolidation. Is sleep necessary for memory reconsolidation? We hypothesized that sleep loss immediately after fear reactivation (FR) will prevent memory reconsolidation. To test our hypothesis, two experiments were performed in adult male C57BL/6J mice exposed to contextual fear conditioning paradigm with inescapable foot shock as unconditional stimulus (US) and contextual cage as conditional stimulus (CS). Sleep loss was achieved either by 5 h of sleep deprivation (SD; Experiment 1) or by systemic infusion of modafinil (200 mg/Kg, ip), an FDA approved wake-promoting agent (Experiment 2). One hour after light-onset, fear memory acquisition (FMA) was performed on Day 1. Mice were allowed to explore CS for 5 min followed by presentation of US (7 foot-shocks; 0.5 mA, 2.0 s duration) at pseudorandom intervals. Controls were exposed to similar CS but no shocks were delivered. On Day 2, mice were exposed to CS for 2 min (without US; for FR) followed by either sleep loss or no sleep loss. On Day 3, fear memory recall (FMR) was performed by exposing mice to CS (without US) for 12 min. Percent time spent in freezing was monitored during FC, FR and FMR. Our results suggested that as compared to sleeping controls, mice with sleep loss immediately after FR displayed a significant reduction in percent time freezing during FMR. These results suggest that sleep loss may prevent memory reconsolidation.

中文翻译:

重新启动恐惧记忆后,立即失去睡眠,这会减轻恐惧记忆的整合。

永久存储的内存通过称为重新激活的过程变得不稳定。一旦重新激活,这些记忆就需要重新整合才能永久保存。睡眠对于内存整合至关重要。睡眠对于重新整合记忆是否必要?我们假设恐惧激活(FR)后立即失眠会阻止记忆重建。为了检验我们的假设,在暴露于情境恐惧条件范式的成年雄性C57BL / 6J小鼠中进行了两个实验,不可避免的足部震荡作为无条件刺激(US),而情境笼作为条件刺激(CS)。睡眠不足可通过剥夺睡眠5小时(SD;实验1)或全身注射FDA批准的促醒剂莫达非尼(200 mg / Kg,ip)(实验2)来实现。发光一小时后 在第1天进行恐惧记忆获取(FMA)。允许小鼠探查CS 5分钟,然后以伪随机间隔进行US(7足电击; 0.5 mA,持续时间为2.0 s)。对照暴露于类似的CS,但未产生电击。在第2天,将小鼠暴露于CS 2分钟(无US;对于FR),然后失眠或无失眠。在第3天,通过将小鼠暴露于CS(无US)12分钟来执行恐惧记忆回忆(FMR)。在FC,FR和FMR期间监控冻结时间所占的百分比。我们的结果表明,与睡眠对照组相比,FR后立即失眠的小鼠表现出FMR冻结时间百分比显着降低。这些结果表明,睡眠不足可能会阻止记忆整合。允许小鼠探索CS 5分钟,然后以伪随机间隔出现US(7次脚震; 0.5 mA,持续时间为2.0 s)。对照暴露于类似的CS,但未产生电击。在第2天,将小鼠暴露于CS 2分钟(无US;对于FR),然后失眠或无失眠。在第3天,通过将小鼠暴露于CS(无US)12分钟来执行恐惧记忆回忆(FMR)。在FC,FR和FMR期间监控冻结时间所占的百分比。我们的结果表明,与睡眠对照组相比,FR后立即失眠的小鼠表现出FMR冻结时间百分比显着降低。这些结果表明,睡眠不足可能会阻止记忆整合。允许小鼠探索CS 5分钟,然后以伪随机间隔出现US(7次脚震; 0.5 mA,2.0 s持续时间)。对照暴露于类似的CS中,但没有电击。在第2天,将小鼠暴露于CS 2分钟(无US;对于FR),然后失眠或无失眠。在第3天,通过将小鼠暴露于CS(无US)12分钟来执行恐惧记忆回忆(FMR)。在FC,FR和FMR期间监控冻结时间所占的百分比。我们的结果表明,与睡眠对照组相比,FR后立即失眠的小鼠表现出FMR冻结时间百分比显着降低。这些结果表明,睡眠不足可能会阻止记忆整合。对照暴露于类似的CS,但未产生电击。在第2天,将小鼠暴露于CS 2分钟(无US;对于FR),然后失眠或无失眠。在第3天,通过将小鼠暴露于CS(无US)12分钟来执行恐惧记忆回忆(FMR)。在FC,FR和FMR期间监控冻结时间所占的百分比。我们的结果表明,与睡眠对照组相比,FR后立即失眠的小鼠表现出FMR冻结时间百分比显着降低。这些结果表明,睡眠不足可能会阻止记忆整合。对照暴露于类似的CS,但未产生电击。在第2天,将小鼠暴露于CS 2分钟(无US;对于FR),然后失眠或无失眠。在第3天,通过将小鼠暴露于CS(无US)12分钟来执行恐惧记忆回忆(FMR)。在FC,FR和FMR期间监控冻结时间所占的百分比。我们的结果表明,与睡眠对照组相比,FR后立即失眠的小鼠表现出FMR冻结时间百分比显着降低。这些结果表明,睡眠不足可能会阻止记忆再巩固。在FC,FR和FMR期间监控冻结时间所占的百分比。我们的结果表明,与睡眠对照组相比,FR后立即失眠的小鼠表现出FMR冻结时间百分比显着降低。这些结果表明,睡眠不足可能会阻止记忆整合。在FC,FR和FMR期间监控冻结时间所占的百分比。我们的结果表明,与睡眠对照组相比,FR后立即失眠的小鼠表现出FMR冻结时间百分比显着降低。这些结果表明,睡眠不足可能会阻止记忆整合。
更新日期:2020-01-07
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