Purple carrots, the original domesticated carrots, accumulate highly glycosylated and acylated anthocyanins in root and/or petiole. Previously, a quantitative trait locus (QTL) for root‐specific anthocyanin pigmentation was genetically mapped to chromosome 3 of carrot. In this study, an R2R3‐MYB gene, namely DcMYB113 , was identified within this QTL region. DcMYB113 expressed in the root of ‘Purple haze’, a carrot cultivar with purple root and nonpurple petiole, but not in the roots of two carrot cultivars with a purple root and petiole (Deep purple and Cosmic purple) and orange carrot ‘Kurodagosun’, which appeared to be caused by variation in the promoter region. The function of DcMYB113 from ‘Purple haze’ was verified by transformation in ‘Cosmic purple’ and ‘Kurodagosun’, resulting in anthocyanin biosynthesis. Transgenic ‘Kurodagosun’ carrying DcMYB113 driven by the CaMV 35S promoter had a purple root and petiole, while transgenic ‘Kurodagosun’ expressing DcMYB113 driven by its own promoter had a purple root and nonpurple petiole, suggesting that root‐specific expression of DcMYB113 was determined by its promoter. DcMYB113 could activate the expression of DcbHLH3 and structural genes related to anthocyanin biosynthesis. DcUCGXT1 and DcSAT1 , which were confirmed to be responsible for anthocyanins glycosylation and acylation, respectively, were also activated by DcMYB113. The WGCNA identified several genes co‐expressed with anthocyanin biosynthesis and the results indicated that DcMYB113 may regulate anthocyanin transport. Our findings provide insight into the molecular mechanism underlying root‐specific anthocyanin biosynthesis and further modification in carrot and even other root crops.

中文翻译：

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DcMYB113是一种根特异性R2R3-MYB，可调节胡萝卜中花色苷的生物合成和修饰。

紫色胡萝卜是原始的驯化胡萝卜，在根和/或叶柄中积累高度糖基化和酰化的花青素。以前，用于根特定花色苷色素沉着的定量性状位点（QTL）已通过基因定位到胡萝卜的3号染色体。在这项研究中，在该QTL区域内鉴定出一个R2R3-MYB基因DcMYB113。DcMYB113在“紫色混浊”的根中表达，“紫色混浊”是具有紫色根和非紫色叶柄的胡萝卜品种，但在两个带有紫色根和叶柄（深紫色和宇宙紫色）和橙色胡萝卜“ Kurodagosun”的胡萝卜品种的根中却没有表达，这似乎是由启动子区域的变化引起的。DcMYB113的功能通过“宇宙紫色”和“ Kurodagosun”中的转化验证了来自“紫色雾霾”的提取物，从而导致了花青素的生物合成。转基因“Kurodagosun”携带DcMYB113由CaMV 35S启动子驱动的过紫色根和叶柄，而转基因“Kurodagosun”表达DcMYB113由它自己的启动子驱动的过紫色根和nonpurple叶柄，提示该根特异性表达DcMYB113通过确定它的启动子。DcMYB113能激活的表达DcbHLH3以及与花青素合成结构基因。DcUCGXT1和DcSAT1DcMYB113也激活了分别被证实分别负责花色苷糖基化和酰化的蛋白。WGCNA鉴定了与花青素生物合成共表达的几个基因，结果表明DcMYB113可能调节花青素的转运。我们的发现为深入了解根特定花色苷生物合成和进一步修饰胡萝卜甚至其他块根作物的分子机制提供了见识。