In the present study, a type of bioconjugate was synthesized by post modification of alginate by conjugating temperature-responsive poly(ε-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(ε-caprolactone-co-lactide) and O-phosphorylethanolamine as phosphorylation functional groups. Freely flowing bioconjugate sols at low temperature can transform to stable viscoelastic gels at the physiological temperature (37 °C). Subcutaneous administration of temperature-responsive bioconjugate sols into the dorsal region of Sprague-Dawley rats formed in situ hydrogel. in situ formation of bioconjugate gels in stimulated body fluids at 37 °C showed nucleation and hydroxyapatite mineral growth. Furthermore, hydroxyapatite growth was also found in in vivo gels, which suggested the potential of alginate-based bioconjugate gels as a scaffold for bone engineering. Bone morphogenetic protein 2 (BMP-2)-loaded bioconjugate formed stable gel in vivo, and demonstrated sustained release. BMP-2-loaded bioconjugates exhibited in situ biomineralization in vivo. These results imply that the in situ formation of injectable biomimetic materials has potential for bone tissue engineering applications.

中文翻译：

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模块化工程设计的藻酸盐生物共轭水凝胶作为生物相容性可注射支架，用于原位生物矿化。

在本研究中，通过对温度响应型聚（ε-己内酯-丙交酯）-b-聚乙二醇-b-聚（ε-己内酯-co-丙交酯）和O-磷酰基乙醇胺作为磷酸化官能团。低温下自由流动的生物共轭溶胶可以在生理温度（37°C）下转变为稳定的粘弹性凝胶。皮下注射温度反应性生物共轭物溶胶到原位水凝胶形成的Sprague-Dawley大鼠的背部区域。在37°C刺激的体液中原位形成生物共轭凝胶显示成核和羟基磷灰石矿物生长。此外，在体内凝胶中还发现了羟基磷灰石的生长，这表明基于藻酸盐的生物缀合物凝胶作为骨工程支架的潜力。加载骨形态发生蛋白2（BMP-2）的生物共轭物在体内形成稳定的凝胶，并显示出持续释放。载有BMP-2-的生物缀合物在体内表现出原位生物矿化作用。这些结果暗示可注射仿生材料的原位形成具有骨组织工程应用的潜力。