Here, we report a facile one-step solid-state reaction assisted synthesis of β-NaFeO₂ perovskite for simultaneous sensing of Dopamine (DA), Uric Acid (UA), Xanthine (Xn) and Hypoxanthine (Hxn) in human blood. The orthorhombic phase formation in β-NaFeO₂ with the presence of octahedral sites is confirmed through x-ray diffraction (XRD) and Raman spectroscopy while high surface area pebble-like morphology is observed through scanning electron microscopy (SEM). The sensor exhibits distinct oxidation potentials for DA, UA, Xn and Hxn with a peak separation (ΔEp) between DA-UA, UA-Xn and Xn-Hxn as 134 mV, 388 mV and 360 mV, respectively. The sensor exhibits an excellent selectivity, sensitivity and low limits of detection (LOD) of 2.12 nM, 158 nM, 129 nM and 95 nM for DA, UA, Xn and Hxn, respectively which are well below the lower limits of their presence in physiological ranges in human body fluids. The sensor shows an excellent selectivity and it was successfully employed in simultaneous sensing of DA, UA, Xn and Hxn in simulated blood serum samples with excellent recovery percentages. This is the first report on low-cost β-NaFeO₂ modified GCE for simultaneous electrochemical sensing of biomolecules which can be applied for numerous bioanalytical applications.

这里，我们报告辅助β-NaFeO的合成的简便一步法固态反应₂钙钛矿对于多巴胺（DA），尿酸（UA），黄嘌呤（XN）和次黄嘌呤（HXN）在人体血液中的同时感测。斜方晶相的形成中β-NaFeO ₂通过X射线衍射（XRD）和拉曼光谱法确认存在八面体位点，而通过扫描电子显微镜（SEM）观察到高表面积的卵石状形态。该传感器对DA，UA，Xn和Hxn表现出不同的氧化电位，在DA-UA，UA-Xn和Xn-Hxn之间的峰间距（ΔEp）分别为134 mV，388 mV和360 mV。该传感器对DA，UA，Xn和Hxn分别具有2.12 nM，158 nM，129 nM和95 nM的优异选择性，灵敏度和低检测限（LOD），远低于其在生理条件下的存在下限人体液体的范围。该传感器具有出色的选择性，已成功用于同时检测模拟血清样品中的DA，UA，Xn和Hxn，回收率极高。_2个改良的GCE，可同时对生物分子进行电化学传感，可用于多种生物分析应用。