Chronic wounds constitute a serious public health problem, and developing pharmaceutical dosage forms to ensure patient comfort and safety, as well as optimizing treatment effectiveness, are of great interest in the pharmaceutical, medical and biomaterial fields. In this work, the preparation of films based on blends of poly(vinyl alcohol), starch and poly(acrylic acid), polymers widely used as pharmaceutical excipients, and pomegranate peel extract (PPE), a bioactive compound with antimicrobial and healing activities relevant to the use as a bioactive wound dressing, was proposed. Initially, the minimum inhibitory concentration (MIC) of the PPE was investigated by an in vitro method. Then, the best concentration of the PPE to be used to prepare the films was researched using an antimicrobial susceptibility test with the disc diffusion method. The microbiological assay was performed in films prepared by the solvent casting method in the presence of two concentrations of PPE: 1.25% w/v and 2.5% w/v. Films containing the lower PPE concentration showed antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis, with a difference that was not considered statistically significant when compared to the higher concentration of the extract. Therefore, the films prepared with the lower proportion of PPE (1.25% w/v) were considered for the other studies. The miscibility and stability of the extract in the films were investigated by thermal analysis. Parameters that determine the barrier properties of the films were also investigated by complementary techniques. Finally, in vitro biological tests were performed for safety evaluation and activity research. Analysis of the results showed that the incorporation of the higher proportion of starch in the blend (15% v/v) (PVA:S:PAA:PPE4) yielded smooth, transparent, and domain-free films without phase separation. Additionally, the PVA:S:PAA:PPE4 film presented barrier properties suitable for use as a cover. These films, when subjected to the in vitro hemolytic activity assay, were nonhemolytic and biocompatible. No toxicity from the extract was observed at the concentrations studied. The results of the wound healing in vitro test showed that films containing 1.25% PPE are efficient in reducing the scratch open area, provoking almost total closure of the scratches within 48 h without cytotoxicity.

慢性伤口构成严重的公共卫生问题，开发药物剂型以确保患者的舒适度和安全性以及优化治疗效果，在药物，医学和生物材料领域引起了极大的兴趣。在这项工作中，基于聚乙烯醇，淀粉和聚丙烯酸的混合物，被广泛用作药物赋形剂的聚合物以及石榴皮提取物（PPE）（一种具有抗微生物和修复活性的生物活性化合物）制备薄膜有人提出将其用作生物活性伤口敷料。最初，通过体外研究了PPE的最低抑菌浓度（MIC）方法。然后，使用抗微生物药敏试验和圆盘扩散法研究了用于制备薄膜的PPE的最佳浓度。在两种浓度的PPE：1.25％w / v和2.5％w / v的存在下，在通过溶剂流延法制备的薄膜中进行微生物分析。含有较低PPE浓度的薄膜对金黄色葡萄球菌和表皮葡萄球菌具有抗微生物活性，与较高浓度提取物相比，该差异被认为没有统计学意义。因此，以较低的PPE（1.25％w / v）用于其他研究。通过热分析研究了提取物在膜中的混溶性和稳定性。还通过补充技术研究了确定薄膜阻隔性能的参数。最后，进行了体外生物学测试，用于安全性评估和活性研究。结果分析表明，在混合物中掺入较高比例的淀粉（15％v / v）（PVA：S：PAA：PPE4）可获得光滑，透明和无畴的膜，没有相分离。另外，PVA：S：PAA：PPE4薄膜具有适合用作覆盖层的阻隔性能。这些薄膜在体外使用时溶血活性测定，具有非溶血性和生物相容性。在所研究的浓度下未观察到提取物的毒性。伤口愈合体外测试的结果表明，含有1.25％PPE的薄膜可有效减少划痕的开口面积，从而在48小时内几乎完全封闭划痕而无细胞毒性。