Based on the concept of LEGO toys, a fiber probe analytical platform (FPAP) was developed as a powerful diagnostic tool offering higher sensitivity in detection of infectious agents compared to established methods. Using the form and the function of LEGO toys, this protocol describes a fiber-based, 96-well plate, which suspends a new class of chemically-designed, electrospun fibers within the assay. This clamping strategy allows both sides of the developed fiber mats to interact with biomolecules within the assay thus benefiting from the tailored chemical and physical properties of these fiber-based bioreceptors in attracting the biomolecules to the surface. The fabrication method of FPAP involves one-step electrospinning of the chemically designed fibers, 3D printing of the LEGO-like probing segments, and assembly of the device followed by ELISA procedure. FPAP follows the same principles of operation as that of a conventional enzyme linked immunosorbent assay (ELISA), therefore, it can be run by lab technicians, expert in ELISA. FPAP was used for early diagnosis of Dengue fever and provided an 8-fold higher sensitivity while the limit of detection (LOD) was recorded to be in femto-gram per milliliter range which is significantly low when compared to other existing techniques or conventional assay. This platform allows different types of paper/fiber bio-receptive platforms to be incorporated within the design that promises simultaneous recognition of multiple infectious agents.

根据乐高玩具的概念，开发了一种纤维探针分析平台（FPAP）作为功能强大的诊断工具，与已建立的方法相比，该方法在检测传染原方面具有更高的灵敏度。该协议使用LEGO玩具的形式和功能，描述了一种基于纤维的96孔板，该板可将一类经过化学设计的新型电纺纤维悬浮在测定中。这种夹持策略允许发达的纤维垫的两侧与化验中的生物分子相互作用，从而受益于这些基于纤维的生物受体在将生物分子吸引到表面上的定制化学和物理特性。FPAP的制造方法包括化学设计纤维的一步电纺，类乐高探针的3D打印，组装设备，然后进行ELISA程序。FPAP遵循与常规酶联免疫吸附测定（ELISA）相同的操作原理，因此，它可以由ELISA专家的实验室技术人员进行操作。FPAP用于登革热的早期诊断，灵敏度提高了8倍，而检测限（LOD）则以毫微克/毫升为单位记录，与其他现有技术或常规测定相比，该值明显偏低。该平台允许将不同类型的纸张/纤维生物接收平台整合到设计中，从而可以同时识别多种传染原。ELISA专家。FPAP用于登革热的早期诊断，灵敏度提高了8倍，而检测限（LOD）则以毫微克/毫升为单位记录，与其他现有技术或常规测定相比，该值明显偏低。该平台允许将不同类型的纸张/纤维生物接收平台整合到设计中，从而可以同时识别多种传染原。ELISA专家。FPAP用于登革热的早期诊断，灵敏度提高了8倍，而检测限（LOD）则以毫微克/毫升为单位记录，与其他现有技术或常规测定相比，该值明显偏低。该平台允许将不同类型的纸张/纤维生物接收平台整合到设计中，从而可以同时识别多种传染原。