Different blood groups of ABO system have specific antigen which bestows them with different biochemical properties and hence they can show different hemolytic activity. In this report hemolytic activity of thiol-functionalized Fe₃O₄-Au nanoparticles was studied in presence and absence of doxorubicin and the effect of various thiol coatings were correlated towards their hemolysis tendency. The nanoparticles were functionalized with four different amino thiols, cysteamine (CEA), cystamine (CA), cysteine (Cys) and cystine (Cyt) to form Fe₃O₄-Au CEA, Fe₃O₄-Au CA, Fe₃O₄-Au Cys and Fe₃O₄-Au Cyt nanoparticles which were loaded with anticancer drug, doxorubicin. The functionalization was characterized using ATR-FTIR, HR-TEM, XPS and other spectroscopic methods. Maximum drug encapsulation efficiency of 83% was observed with Fe₃O₄-Au CA nanoparticles. In-vitro experiments were performed on HeLa to check the cellular uptake and cytotoxicity using MTT assay. Hemolytic activity was then analyzed with all the blood groups (positive and negative). The amino acid functionalized, Fe₃O₄-Au Cys and Fe₃O₄-Au Cyt nanoparticles, show lesser hemolysis compared to amino thiol functionalized Fe₃O₄-Au CEA, and Fe₃O₄-Au CA nanoparticles. In positive blood groups, the Fe₃O₄-Au CA nanoparticles shows the highest rate of hemolysis followed by Fe₃O₄-Au CEA, while the lowest hemolysis rate was observed for Fe₃O₄-Au Cyt nanoparticles. For negative blood groups, the thiol coated nanoparticles show more abrupt hemolysis rate depending upon the type of antigen.

ABO系统的不同血型具有特定的抗原，赋予它们不同的生化特性，因此它们可以表现出不同的溶血活性。在该报告中，研究了在存在和不存在阿霉素的情况下，巯基官能化的Fe ₃ O ₄ -Au纳米颗粒的溶血活性，并且各种巯基涂层的作用与其溶血趋势相关。用四种不同的氨基硫醇，半胱胺（CEA），胱胺（CA），半胱氨酸（Cys）和胱氨酸（Cyt）对纳米颗粒进行功能化，以形成Fe ₃ O ₄ -Au CEA，Fe ₃ O ₄ -Au CA，Fe ₃ O ₄ -Au Cys和Fe ₃ O ₄-载有抗癌药阿霉素的Au Cyt纳米颗粒。使用ATR-FTIR，HR-TEM，XPS和其他光谱方法对功能化进行了表征。Fe ₃ O ₄ -Au CA纳米颗粒的最大药物封装效率为83％。使用MTT分析在HeLa上进行了体外实验，以检查细胞摄取和细胞毒性。然后分析所有血型（阳性和阴性）的溶血活性。氨基酸官能化，铁_3个ö _4点-Au的Cys和Fe ₃ Ò _4个相比-Au细胞色素纳米颗粒，显示出较小的溶血氨基硫醇官能化的Fe ₃ ö ₄ -Au CEA，和Fe ₃O ₄ -Au CA纳米颗粒。在阳性血型中，Fe ₃ O ₄ -Au CAn纳米颗粒的溶血率最高，其次是Fe ₃ O ₄ -Au CEA，而Fe ₃ O ₄ -Au Cyt纳米颗粒的溶血率最低。对于阴性血型，根据抗原类型，巯基包裹的纳米颗粒表现出更突然的溶血速率。