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Structural and magnetic characteristics of carboxymethyl dextran coated magnetic nanoparticles: From characterization to immobilization application
Reactive & Functional Polymers ( IF 5.1 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.reactfunctpolym.2020.104481
Katja Vasić , Željko Knez , Elizaveta A. Konstantinova , Alexander I. Kokorin , Sašo Gyergyek , Maja Leitgeb

MNPs were synthesized with co-precipitation of ferrous and ferric ions. Carboxymethyl dextran (CMD) was covalently bound to MNPs and the influence of different concentrations on characteristics of CMD coated magnetic nanoparticles (CMD-MNPs) was studied. Different concentrations of CMD were applied into synthesis process. The surface morphology of CMD-MNPs was monitored by scanning and transmission electron microscopy, where their spherical shape was confirmed. Fourier transform infrared spectroscopy displayed characteristic bonds that confirmed the presence of CMD hydroxyl and carboxyl groups. Thermogravimetric analysis displayed a weight loss, which confirmed the coating weight of CMD. ζ-potential measurements revealed negatively charged hydroxyl groups of CMD, and polydispersity index (PdI) showed the most consistent sizes of CMD3-MNPs. Electron paramagnetic resonance and magnetization measurements confirmed a ferromagnetic system for all CMD-MNPs. Prepared CMD3-MNPs were used as carriers for immobilization of enzyme alcohol dehydrogenase (ADH). Immobilization was carried out at two different temperatures (20 °C and 4 °C) and thermal stability at 20 °C and 40 °C after 24 h was studied. Prepared CMD-MNPs exhibit a layer of CMD coating that provides proper magnetic and structural properties and can therefore be functionalized and used in bioactive compound immobilization, such as ADH.



中文翻译:

羧甲基葡聚糖包被的磁性纳米颗粒的结构和磁性特征:从表征到固定化应用

MNP是通过铁和铁离子的共沉淀而合成的。羧甲基葡聚糖(CMD)与MNP共价结合,研究了不同浓度对CMD包覆的磁性纳米颗粒(CMD-MNPs)特性的影响。将不同浓度的CMD应用于合成过程。通过扫描和透射电子显微镜监测CMD-MNPs的表面形态,确认其球形。傅里叶变换红外光谱显示出特征键,这些键确认了CMD羟基和羧基的存在。热重分析显示出重量损失,这证实了CMD的涂层重量。ζ电位测量显示CMD带负电的羟基,多分散指数(PdI)显示CMD3-MNP的尺寸最一致。电子顺磁共振和磁化强度测量结果证实了所有CMD-MNP的铁磁系统。制备的CMD3-MNPs被用作固定化酶乙醇脱氢酶(ADH)的载体。固定在两个不同的温度(20°C和4°C)下进行,研究了24小时后在20°C和40°C下的热稳定性。制备的CMD-MNP表现出一层CMD涂层,可提供适当的磁性和结构特性,因此可以功能化并用于固定生物活性化合物(如ADH)。固定在两个不同的温度(20°C和4°C)下进行,研究了24小时后在20°C和40°C下的热稳定性。制备的CMD-MNP表现出一层CMD涂层,可提供适当的磁性和结构特性,因此可以功能化并用于固定生物活性化合物(如ADH)。固定在两个不同的温度(20°C和4°C)下进行,研究了24小时后在20°C和40°C下的热稳定性。制备的CMD-MNP表现出一层CMD涂层,可提供适当的磁性和结构特性,因此可以功能化并用于固定生物活性化合物(如ADH)。

更新日期:2020-01-07
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