Abstract Lycopene is an antioxidant that can be used as functional food or a high-value product for nutraceutical, pharmaceutical, and cosmetic applications. In this paper, the mevalonate and lycopene synthesis pathways were first built in Escherichia coli to produce lycopene from hydrophilic substrates such as glucose and glycerol. Further introducing the fatty acid transport system in the E. coli strain led to improved synthesis of lycopene from hydrophobic substrates such as fatty acids. In 1-L fed-batch bioreactors, the engineered E. coli FA03-PM produced 2.7 g/L lycopene within 40 h in fermentation co-fed with glucose and hydrolyzed waste cooking oil (WCO). This is the first study that reports high lycopene production by fermentation with fatty acids or waste cooking oils. In addition, the lycopene content in biomass reached 94 mg/g, which is among the highest levels reported so far in engineered E. coli for lycopene production. The research results will also help build a new biomanufacturing platform for making more high-value products from the common agriculture commodities, oils and fats, or their wastes.

中文翻译：

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代谢工程大肠杆菌从葡萄糖、脂肪酸和废食用油中生产番茄红素

摘要 番茄红素是一种抗氧化剂，可用作功能性食品或营养保健品、药物和化妆品应用的高价值产品。在本文中，甲羟戊酸和番茄红素合成途径首先在大肠杆菌中构建，以从葡萄糖和甘油等亲水性底物生产番茄红素。在大肠杆菌菌株中进一步引入脂肪酸转运系统，可以改善由脂肪酸等疏水性底物合成番茄红素。在 1-L 分批补料生物反应器中，工程大肠杆菌 FA03-PM 在与葡萄糖和水解废食用油 (WCO) 共同发酵的 40 小时内产生 2.7 g/L 番茄红素。这是第一项报告通过用脂肪酸或废弃食用油发酵产生高番茄红素的研究。此外，生物质中番茄红素含量达到94mg/g，这是迄今为止在用于番茄红素生产的工程大肠杆菌中报道的最高水平之一。研究成果还将有助于建立一个新的生物制造平台，以利用常见的农产品、油脂或它们的废物制造更多高价值产品。