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Neurochemical Organization of the Drosophila Brain Visualized by Endogenously Tagged Neurotransmitter Receptors.
Cell Reports ( IF 8.8 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.celrep.2019.12.018
Shu Kondo 1 , Takahiro Takahashi 2 , Nobuhiro Yamagata 2 , Yasuhito Imanishi 2 , Hidetaka Katow 1 , Shun Hiramatsu 2 , Katrina Lynn 2 , Ayako Abe 2 , Ajayrama Kumaraswamy 3 , Hiromu Tanimoto 2
Affiliation  

Neurotransmitters often have multiple receptors that induce distinct responses in receiving cells. Expression and localization of neurotransmitter receptors in individual neurons are therefore critical for understanding the operation of neural circuits. Here we describe a comprehensive library of reporter strains in which a convertible T2A-GAL4 cassette is inserted into endogenous neurotransmitter receptor genes of Drosophila. Using this library, we profile the expression of 75 neurotransmitter receptors in the brain. Cluster analysis reveals neurochemical segmentation of the brain, distinguishing higher brain centers from the rest. By recombinase-mediated cassette exchange, we convert T2A-GAL4 into split-GFP and Tango to visualize subcellular localization and activation of dopamine receptors in specific cell types. This reveals striking differences in their subcellular localization, which may underlie the distinct cellular responses to dopamine in different behavioral contexts. Our resources thus provide a versatile toolkit for dissecting the cellular organization and function of neurotransmitter systems in the fly brain.

中文翻译:

果蝇大脑的神经化学组织通过内源性标记的神经递质受体可视化。

神经递质通常具有多种受体,这些受体在接受细胞中诱导不同的反应。因此,神经递质受体在单个神经元中的表达和定位对于理解神经回路的运作至关重要。在这里,我们描述了一个完整的报告菌株库,其中可转换的T2A-GAL4盒插入果蝇的内源性神经递质受体基因中。使用此库,我们可以分析大脑中75种神经递质受体的表达。聚类分析揭示了大脑的神经化学分割,从而将较高的大脑中枢与其他区域区分开。通过重组酶介导的盒式交换,我们将T2A-GAL4转换为split-GFP和Tango,以可视化特定细胞类型中的亚细胞定位和多巴胺受体的激活。这揭示了它们亚细胞定位的显着差异,这可能是在不同行为情况下对多巴胺的不同细胞反应的基础。因此,我们的资源提供了用于剖析苍蝇大脑中神经递质系统的细胞组织和功能的通用工具包。
更新日期:2020-01-07
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