Three new pentacyclic triterpenoid saponins (1-3), together with medicagenic acid (4) were isolated and purified from 70% EtOH extract of Medicago sativa L. by different column chromatographic and semi-preparative HPLC. Their structures were established by direct interpretation of their spectral data, mainly HR-ESI-MS, 1D-NMR, 2D-NMR, and chemical methods, as well as comparison with literature data. Additionally, all isolates were evaluated for their neuroprotective activities against H2O2-induced damage in human neuroblastoma SHSY5Y cells. As a results, compounds 1 and 2 (67.14% and 73.05%) exhibited potent neuroprotective activities. These findings provide new insights into developing better treatment of neurodegenerative diseases for M. sativa in the future.

中文翻译：

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具有神经保护活性的紫花苜蓿中三萜皂苷的分离。

通过不同柱色谱和半制备性HPLC从70％紫花苜蓿的EtOH提取物中分离和纯化了三种新的五环三萜皂苷（1-3）和药用酸（4）。通过直接解释其光谱数据（主要是HR-ESI-MS，1D-NMR，2D-NMR和化学方法）以及与文献数据进行比较来建立其结构。此外，评估了所有分离株对人类神经母细胞瘤SHSY5Y细胞中H2O2诱导的损伤的神经保护活性。结果，化合物1和2（67.14％和73.05％）表现出有效的神经保护活性。这些发现为将来开发更好的苜蓿神经退行性疾病治疗提供了新的见识。