A novel and green spectrophotometric method, namely constant extraction, based on extracting a spectral feature value (constant ratio) has been developed and validated for simultaneous estimation of a binary mixture of zofenopril calcium (ZOF) and hydrochlorothiazide (HCT) in their bulk and marketable formulation. A comparative study between this newly developed method and four long-established ones; ratio difference, ratio subtraction coupled with constant multiplication, advanced amplitude modulation and absorbance subtraction has been carried out giving very promising results. Various analytical performance parameters like linearity, accuracy, precision, and robustness were investigated in accordance with ICH (Q2B). Satisfying optimized instrumental parameters; absorbance of ZOF and HCT were linearly increased for the previously mentioned methods in a concentration range 5.0-35.0 and 3.0-20.0 μg/mL, respectively showing correlation coefficients ≥0.9990. Moreover, specificity has been checked through analyzing synthetic mixtures of the studied analytes. Feasibility has been successfully assessed by simultaneous quantification of both analytes in the commercially available formulation. As well, validity was examined and no interference from common excipients was noticed. Observed data has been statistically compared with those of the published one concluding that no significant variations between both results have been indicated. Furthermore, greenness profile of these methods was assessed by analytical Eco-Scale and found superior to that of the reported HPLC method as an even greener approach for the simultaneous analysis of ZOF and HCT.

中文翻译：

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用于实现潜在光谱分辨率的新颖特征提取方法：佐芬普利钙和氢氯噻嗪在光谱重叠的二元混合物中的绿色分析应用。

已经开发了一种新颖的绿色分光光度法，即基于提取光谱特征值（恒定比率）的恒定提取，并同时用于批量估计佐芬普利钙（ZOF）和氢氯噻嗪（HCT）的二元混合物，并且可市售公式。这种新开发的方法与四种老方法之间的比较研究；比率差，比率减法与常数相乘，先进的幅度调制和吸收度减法相结合已经取得了令人鼓舞的结果。根据ICH（Q2B），研究了各种分析性能参数，如线性，准确性，精确度和鲁棒性。满足优化的仪器参数；在浓度范围为5.0-35.0和3.0-20.0μg/ mL的情况下，前述方法中ZOF和HCT的吸光度呈线性增加，相关系数≥0.9990。此外，已经通过分析所研究的分析物的合成混合物来检查特异性。通过同时定量市售制剂中的两种分析物已成功评估了可行性。同样，检查了有效性，没有发现来自普通赋形剂的干扰。将观察到的数据与已发表的数据进行了统计比较，得出的结论是两种结果之间均未发现明显差异。此外，