A novel, mechano-biocatalytic one-pot process was developed by this study to efficiently release monosaccharides from lignocellulosic materials in an environmentally-friendly manner. The process synergistically integrates ball milling and enzymatic hydrolysis to complete pretreatment and hydrolysis of lignocellulosic materials in a single step without chemical supplements. High sugar titer and conversion from lignocellulosic materials were simultaneously achieved. Among four studied feedstocks (solid digestate, corn stover, switchgrass, and miscanthus), corn stover demonstrated much better sugar concentration and conversion. Under the preferred reaction condition, the glucose concentration reached 55.20 g/L with a glucose conversion of 88.63%. The corresponding xylose concentration was 20.06 g/L with a xylose conversion of 67.34%. The energy and exergy analyses further indicate that the studied process had better energy and exergy profiles than the conventional combined hydrolysis process. The average energy consumption of the mechano-biocatalytic process for four feedstocks was 1.05 kWh-e/kg dry biomass that was 56% lower than the average energy consumption (2.37 kWh-e/kg dry biomass) of the conventional process. The corresponding average exergy efficiency of the mechano-biocatalytic process was 67% that was much higher than the average efficiency (52%) of the conventional process. These results show that the mechano-biocatalytic one-pot process as an environmentally friendly approach can significantly simplify the pretreatment and hydrolysis and enhance their efficiencies for advanced fuel and chemical production.

这项研究开发了一种新颖的机械生物催化一锅法，以一种环境友好的方式有效地从木质纤维素材料中释放单糖。该工艺将球磨和酶促水解相结合，无需任何化学添加剂即可在一个步骤中完成木质纤维素材料的预处理和水解。同时实现了高糖滴度和木质纤维素材料的转化。在四种研究的原料（固体消化物，玉米秸秆，柳枝switch和桔梗）中，玉米秸秆显示出更好的糖浓度和转化率。在优选的反应条件下，葡萄糖浓度达到55.20g / L，葡萄糖转化率为88.63％。相应的木糖浓度为20.06 g / L，木糖转化率为67.34％。能量和火用分析进一步表明，所研究的过程具有比常规组合水解过程更好的能量和火用曲线。四种原料的机械生物催化过程的平均能耗为1.05 kWh-e / kg干生物质，比常规过程的平均能耗（2.37 kWh-e / kg干生物质）低56％。机械-生物催化过程的相应平均火用效率为67％，远高于常规过程的平均效率（52％）。这些结果表明，机械-生物催化一锅法作为一种环境友好的方法可以显着简化预处理和水解过程，并提高其先进燃料和化学生产的效率。