In this study, effects of different single biomass derived inhibitors on acetone–butanol–ethanol (ABE) production by Clostridium acetobutylicum CICC 8016 were first investigated. The results showed that formic acid, coumaric acid, and furfural at 0.5 g/L (sodium formate equivalent) inhibited ABE production. Furthermore, corn stover hydrolysate media were prepared following dilute acid pretreatment, enzymatic hydrolysis, and detoxification with different methods. Among overliming, steam stripping, acetone–ethyl ether extraction, and ion exchange with five anion resins, adsorption with resin D301 showed the highest efficiency for inhibitor removal (99–100% of phenolics and 87–99% of sugar degradation products). Without detoxification, ABE production was lower than 1.0 g/L from 28.1 g/L sugars whereas ABE production with medium detoxified by D301 resin achieved higher ABE concentrations and yields than control with synthetic medium. Correlation analysis further revealed that formic acid, coumaric acid, and total phenolics were the major compounds inhibiting ABE production. The results also showed that the single detoxification method was sufficient to detoxify the hydrolysate for ABE production at the pretreatment conditions used in this study.

中文翻译：

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排毒方法对丙酮丁醇梭菌CICC 8016玉米秸秆水解产物生产ABE的影响。

在这项研究中，不同的单一生物质衍生抑制剂对丙酮丁醇梭菌生产丙酮-丁醇-乙醇（ABE）的影响首先对CICC 8016进行了研究。结果表明，甲酸，香豆酸和糠醛浓度为0.5 g / L（甲酸钠当量）会抑制ABE的产生。此外，在稀酸预处理，酶水解和用不同方法进行解毒之后，制备了玉米秸秆水解产物培养基。在上胶，蒸汽汽提，丙酮-乙醚萃取以及与五种阴离子树脂的离子交换中，树脂D301的吸附显示出最高的抑制剂去除效率（99-100％的酚类和87-99％的糖降解产物）。没有排毒，ABE的产量低于28.1 g / L糖的1.0 g / L，而用D301树脂排毒的培养基的ABE产量要高于用合成培养基的对照的ABE浓度和产量。相关分析进一步表明，甲酸，香豆酸和总酚是抑制ABE生成的主要化合物。结果还表明，在本研究中使用的预处理条件下，单一解毒方法足以解毒水解产物以生产ABE。