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Up-regulation of circ_LARP4 suppresses cell proliferation and migration in ovarian cancer by regulating miR-513b-5p/LARP4 axis.
Cancer Cell International ( IF 5.8 ) Pub Date : 2020-01-06 , DOI: 10.1186/s12935-019-1071-z
Wumei Lin 1 , Haiyan Ye 1 , Keli You 1 , Le Chen 1
Affiliation  

Background Ovarian cancer (OC) is a common fatal malignant tumor of female reproductive system worldwide. Growing studies have proofed that circular RNAs (circRNAs) engage in the regulation of various types of cancers. However, the underlying biological functions and effect mechanism of circular RNA_LARP4 (circ_LARP4) in OC have not been explored. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to detect the expression of circ_LARP4 in OC cells. The function of circ_LARP4 was measured by cell counting kit-8 (CCK-8), colony formation assay and transwell assay. RNA immunoprecipitation (RIP) assay and luciferase reporter assays assessed the binding correlation between miR-513b-5p and circ_LARP4 (or LARP4). Results The expression of circ_LARP4 in OC cells was much lower than that in human normal ovarian epithelial cells. Overexpressing circ_LARP4 impaired cell proliferation, invasion and migration abilities. Circ_LARP4 worked as a competing endogenous RNA (ceRNA) to sponge miR-513b-5p. Furthermore, LARP4 was indirectly modulated by circ_LARP4 as the downstream target of miR-513b-5p, as well as the host gene of circ_LARP4. Conclusion Circ_LARP4 could hamper cell proliferation and migration by sponging miR-513b-5p to regulate the expression of LARP4. This research may provide some referential value to OC treatment.

中文翻译:

circ_LARP4 的上调通过调节 miR-513b-5p/LARP4 轴来抑制卵巢癌中的细胞增殖和迁移。

背景卵巢癌(OC)是世界范围内女性生殖系统常见的致命性恶性肿瘤。越来越多的研究证明环状 RNA (circRNA) 参与调节各种类型的癌症。然而,环状RNA_LARP4(circ_LARP4)在OC中的潜在生物学功能和作用机制尚未被探索。方法采用定量实时聚合酶链反应(qRT-PCR)分析检测oc细胞中circ_LARP4的表达。circ_LARP4的功能通过细胞计数试剂盒8(CCK-8)、集落形成试验和transwell试验进行测定。RNA 免疫沉淀 (RIP) 分析和荧光素酶报告基因分析评估了 miR-513b-5p 和 circ_LARP4(或 LARP4)之间的结合相关性。结果 circ_LARP4在OC细胞中的表达远低于人正常卵巢上皮细胞。过度表达 circ_LARP4 会损害细胞增殖、侵袭和迁移能力。Circ_LARP4 作为一种竞争性内源性 RNA (ceRNA) 来海绵 miR-513b-5p。此外,LARP4 被 circ_LARP4 作为 miR-513b-5p 的下游靶标以及 circ_LARP4 的宿主基因间接调节。结论 Circ_LARP4可以通过海绵化miR-513b-5p调控LARP4的表达来阻碍细胞增殖和迁移。本研究可为OC治疗提供一定的参考价值。LARP4 被 circ_LARP4 作为 miR-513b-5p 的下游靶点以及 circ_LARP4 的宿主基因间接调节。结论 Circ_LARP4可以通过海绵化miR-513b-5p调控LARP4的表达来阻碍细胞增殖和迁移。本研究可为OC治疗提供一定的参考价值。LARP4 被 circ_LARP4 作为 miR-513b-5p 的下游靶点以及 circ_LARP4 的宿主基因间接调节。结论 Circ_LARP4可以通过海绵化miR-513b-5p调控LARP4的表达来阻碍细胞增殖和迁移。本研究可为OC治疗提供一定的参考价值。
更新日期:2020-01-06
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