We produced a prometryn-specific monoclonal antibody and propose a strategy for convenient on-site detection of prometryn residues in herbs for the first time. This strategy has perfect applicability in a complex herbal medicine matrix. The strategy combines a semiquantitative immunochromatographic strip assay with a heterologous indirect competitive ELISA. When there was no matrix interference, the ELISA had a half-maximal inhibitory concentration of 2.6 ng·mL-1 and a limit of detection of 0.2 ng·mL-1. The immunochromatographic strip assay can be completed within 5 min with a visual limit of detection of 1 ng·mL-1. Although the sample matrix had different effects on the sensitivity of the antibody, excellent repeatability and accuracy were achieved. The method was successfully applied for the screening and determination of prometryn residue in multiple complex herb samples for the first time, and the results were in good agreement with those obtained by liquid chromatography-tandem mass spectrometry. The proposed strategy is rapid, of high-throughput, and of low cost, and may be a promising choice for on-site detection of prometryn in different kinds of herbs. Graphical abstract.

中文翻译：

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一种快速现场筛选和测定草药中脯氨酰胺残留的综合策略。

我们生产了一种prometricn特异性单克隆抗体，并首次提出了一种方便现场检测草药中prometricn残留的策略。该策略在复杂的草药基质中具有完美的适用性。该策略将半定量免疫色谱带分析与异源间接竞争ELISA相结合。当没有基质干扰时，ELISA的最大抑制浓度为2.6 ng·mL-1，检测限为0.2 ng·mL-1。免疫色谱条测定法可在5分钟内完成，视觉检测极限为1 ng·mL-1。尽管样品基质对抗体的敏感性有不同的影响，但仍具有出色的可重复性和准确性。该方法首次成功地用于多种复杂草药样品中脯氨酰胺残留的筛选和测定，结果与液相色谱-串联质谱法相吻合。所提出的策略是快速的，高通量的且低成本的，并且可能是用于现场检测不同种类的草药中的脯氨酸的有前途的选择。图形概要。