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A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair
Nature Structural & Molecular Biology ( IF 16.8 ) Pub Date : 2020-01-06 , DOI: 10.1038/s41594-019-0355-2
Kaiying Cheng 1 , Martin Wilkinson 1 , Yuriy Chaban 2 , Dale B Wigley 1
Affiliation  

The RecBCD complex plays key roles in phage DNA degradation, CRISPR array acquisition (adaptation) and host DNA repair. The switch between these roles is regulated by a DNA sequence called Chi. We report cryo-EM structures of the Escherichia coli RecBCD complex bound to several different DNA forks containing a Chi sequence, including one in which Chi is recognized and others in which it is not. The Chi-recognized structure shows conformational changes in regions of the protein that contact Chi and reveals a tortuous path taken by the DNA. Sequence specificity arises from interactions with both the RecC subunit and the sequence itself. These structures provide molecular details for how Chi is recognized and insights into the changes that occur in response to Chi binding that switch RecBCD from bacteriophage destruction and CRISPR spacer acquisition to constructive host DNA repair.



中文翻译:

响应 Chi 的构象转换将 RecBCD 从噬菌体破坏转变为 DNA 修复

RecBCD 复合物在噬菌体 DNA 降解、CRISPR 阵列采集(适应)和宿主 DNA 修复中起关键作用。这些角色之间的转换由称为 Chi 的 DNA 序列调节。我们报告了大肠杆菌的冷冻电镜结构RecBCD 复合物与几个不同的包含 Chi 序列的 DNA 叉结合,其中一个可以识别 Chi,另一个不识别。Chi 识别的结构显示了与 Chi 接触的蛋白质区域的构象变化,并揭示了 DNA 所走的曲折路径。序列特异性来自与 RecC 亚基和序列本身的相互作用。这些结构为如何识别 Chi 提供了分子细节,并深入了解了响应 Chi 结合而发生的变化,这些变化将 RecBCD 从噬菌体破坏和 CRISPR 间隔子获取转变为建设性宿主 DNA 修复。

更新日期:2020-01-06
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