In this review, the application of CRISPR/Cas9 plant genome editing using alternative transformation methods is discussed. Genome editing by the CRISPR/Cas9 system is usually implemented via the generation of transgenic plants carrying Cas9 and sgRNA genes in the genome. Transgenic plants are usually developed by in vitro regeneration from single transformed cells, which requires using different in vitro culture-based methods. Despite their common application, these methods have some disadvantages and limitations. Thus, some methods of plant transformation that do not depend on in vitro regeneration have been developed. These methods are known as “in planta” transformation. The main focus of this review is the so-called floral dip in planta transformation method, although other approaches are also described. The main features of in planta transformation in the context of CRISPR/Cas9 genome editing are discussed. Furthermore, multiple ways to increase the effectiveness of this approach and to broaden its use in different plant species are considered.

在这篇综述中，讨论了使用替代转化方法的CRISPR / Cas9植物基因组编辑的应用。CRISPR / Cas9系统的基因组编辑通常是通过生成在基因组中携带Cas9和sgRNA基因的转基因植物来实现的。转基因植物通常是通过从单个转化细胞进行体外再生而开发的，这需要使用不同的基于体外培养的方法。尽管它们普遍应用，但是这些方法也有一些缺点和局限性。因此，已经开发了一些不依赖于体外再生的植物转化方法。这些方法在植物中被称为““ 转型。这篇综述的主要重点是所谓的植物浸入法浸入植物转化方法，尽管还介绍了其他方法。讨论了在CRISPR / Cas9基因组编辑的背景下植物转化的主要特征。此外，考虑了多种方法来提高该方法的有效性并扩大其在不同植物物种中的使用。