The anthocyanins contents from red rice bran were characterized by HPLC/MS. Response surface methodology was used to optimize the ultrasound‐assisted extraction of red rice bran anthocyanin. The antioxidant activities were evaluated in terms of IC₅₀. The tyrosinase inhibitory activities of the anthocyanin samples from red rice bran and the standard substances were determined by a spectrophotometric method. According to mass spectrometry information, the main component of anthocyanins is paeoniflorin (m/z = 480). The optimized anthocyanin level was 5.80 mg/g under the following conditions: solid–liquid ratio of 1:17.46; ethanol concentration of 78.37%; ultrasonication time of 55.23 min; and pH of 2.31. The IC₅₀ value of the DPPH radical scavenging and the superoxide anion scavenging activities of the sample were 53.51 and 2,375 μg/ml; those of the standard were 14.60 and 64.74 μg/ml; and those of vitamin C were 24.45 and 136.25 μg/ml, respectively. The IC₅₀ values of the tyrosinase inhibition activities of the sample and Vc were 4.26 and 2.18 μg/ml, respectively. There is a significant difference (p < .05) between the activities of the three, which may be caused by the purity of the extract. Red rice bran anthocyanins have valuable research and development prospects as skin whiteners and healthcare products.

中文翻译：

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通过响应面法，红米糠中花色苷的抗氧化能力和酪氨酸酶抑制活性优化超声波辅助提取

用HPLC / MS对红米糠中花色苷的含量进行了表征。响应面方法被用于优化红米糠花色苷的超声辅助提取。抗氧化活性根据IC ₅₀进行评估。用分光光度法测定了红米糠中的花色苷样品和标准物质中的酪氨酸酶抑制活性。根据质谱信息，花色苷的主要成分是pa药苷（m / z  = 480）。在以下条件下，优化的花色苷水平为5.80 mg / g：固液比为1：17.46；乙醇浓度为78.37％；超声时间为55.23分钟；pH值为2.31。IC ₅₀样品的DPPH自由基清除率和超氧阴离子清除率分别为53.51和2,375μg/ ml。标准浓度分别为14.60和64.74μg/ ml; 维生素C的含量分别为24.45和136.25μg/ ml。样品和Vc的酪氨酸酶抑制活性的IC ₅₀值分别为4.26和2.18μg/ ml。 三种活性之间存在显着差异（p <.05），这可能是由于提取物的纯度所致。红米糠花青素作为皮肤增白剂和保健品具有重要的研发前景。