Abstract Microbial volatiles can promote plant growth and suppress diseases, nematodes and insects. Our knowledge of the effects of microbial volatiles on monocots such as rice and its pathogens and pests is still incomplete. As part of a screening initiative for beneficial bacterial species, we identified three strains (Bacillus sp., Paenibacillus sp. and Xanthomonas sp.) lethal to the rice root-knot nematode (Meloidogyne graminicola). Using both in vitro and in planta dual-chamber pot experiments, we found that volatiles from the beneficial bacteria were lethal to M. graminicola J2s and significantly reduced infection of susceptible rice. We conducted similar in vitro and in planta experiments against rice bacterial leaf blight Xanthomonas oryzae pv. oryzae. Exposure to bacterial volatiles inhibited the growth of X. oryzae pv. oryzae by 50–60% in vitro, but did not impact infection in planta. Finally, we found that bacterial volatiles from Bacillus sp. and Xanthomonas sp. inhibited rice germination and seedling development in vitro but did not affect plant growth in planta. These findings suggest that volatiles from beneficial bacteria have the potential to control M. graminicola, but that high volatile concentrations may inhibit plant growth.

中文翻译：

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防治水稻根结线虫和白叶枯病的有益细菌挥发性化合物

摘要 微生物挥发物具有促进植物生长、抑制病害、线虫和昆虫的作用。我们对微生物挥发物对水稻等单子叶植物及其病原体和害虫的影响的知识仍然不完整。作为有益细菌物种筛选计划的一部分，我们鉴定了三种对水稻根结线虫 (Meloidogyne graminicola) 致死的菌株（芽孢杆菌属、类芽孢杆菌属和黄单胞菌属）。使用体外和植物双室盆栽实验，我们发现有益细菌的挥发物对禾本科 M. graminicola J2s 是致命的，并显着降低了易感水稻的感染。我们进行了类似的体外和植物实验，以对抗水稻细菌性叶枯病 Xanthomonas oryzae pv。稻瘟病菌。暴露于细菌挥发物抑制了 X. oryzae pv 的生长。oryzae 体外 50-60%，但不影响植物的感染。最后，我们发现来自芽孢杆菌的细菌挥发物。和黄单胞菌属。在体外抑制水稻发芽和幼苗发育，但不影响植物生长。这些发现表明，来自有益细菌的挥发物具有控制禾本科禾本科的潜力，但高挥发物浓度可能会抑制植物生长。