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Overexpression of soybean DREB1 enhances drought stress tolerance of transgenic wheat in the field.
Journal of Experimental Botany ( IF 6.9 ) Pub Date : 2019-12-26 , DOI: 10.1093/jxb/erz569
Yongbin Zhou 1 , Ming Chen 2 , Jinkao Guo 3 , Yanxia Wang 3 , Donghong Min 1 , Qiyan Jiang 2 , Hutai Ji 4 , Chengyan Huang 5 , Wei Wei 2 , Huijun Xu 2 , Xiao Chen 2 , Liancheng Li 2 , Zhaoshi Xu 2 , Xianguo Cheng 2 , Chunxiao Wang 2 , Chengshe Wang 1 , Youzhi Ma 2
Affiliation  

DREB (drought response element binding) like transcription factors can significantly enhance plant tolerance to drought. However, most research on DREB-like proteins to date has been conducted in growth chambers or greenhouses, so there is very little evidence available to support the application of DREB-like proteins in the field. Here, we overexpressed GmDREB1 from soybean in two popular wheat varieties and conducted several drought tolerance experiments across a range of years, sites and drought-stress regimes. We found that these plants consistently exhibited significant improvements in yield performance and a variety of physiological traits over WT plants when grown under limited water conditions in the field, for example showing grain yield increases between 4.79-18.43%. Specifically, we found that the transgenic wheat plants had reduced membrane damage and enhanced osmotic adjustment as well photosynthetic efficiency compared to non-transgenic controls. Three enzymes from the biosynthetic pathway of the phytohormone melatonin were up-regulated in transgenic wheat, further supporting a role for melatonin in the observed drought tolerance, external application of melatonin improved drought tolerance performance. Collectively, our results convincingly demonstrate the utility of a transgenic overexpression strategy for using GmDREB1 to improve wheat drought tolerance in the field.

中文翻译:

大豆DREB1的过表达增强了转基因小麦在田间的耐旱性。

像转录因子一样的DREB(干旱反应元件结合)可以显着增强植物对干旱的耐受性。但是,迄今为止,大多数关于DREB样蛋白的研究都是在生长室或温室中进行的,因此很少有证据支持DREB样蛋白在该领域的应用。在这里,我们在两个流行的小麦品种中从大豆中过量表达了GmDREB1,并在多年,地点和干旱胁迫制度的范围内进行了几次耐旱性试验。我们发现这些植物在田间在有限的水分条件下生长时,与野生型植物相比,始终表现出显着的增产性能和各种生理性状,例如谷物产量增加了4.79%至18.43%。特别,我们发现,与非转基因对照相比,转基因小麦植物具有减少的膜损伤和增强的渗透调节以及光合作用效率。来自植物激素褪黑激素生物合成途径的三种酶在转基因小麦中上调,进一步支持褪黑激素在观察到的抗旱性中的作用,褪黑激素的外部施用改善了抗旱性。总的来说,我们的结果令人信服地证明了使用GmDREB1改善田间小麦耐旱性的转基因过表达策略的实用性。褪黑激素进一步支持了褪黑激素在观察到的抗旱性中的作用,褪黑激素的外部施用改善了抗旱性。总的来说,我们的结果令人信服地证明了使用GmDREB1改善田间小麦耐旱性的转基因过表达策略的实用性。褪黑激素进一步支持了褪黑激素在观察到的抗旱性中的作用,褪黑激素的外部施用改善了抗旱性。总的来说,我们的结果令人信服地证明了使用GmDREB1改善田间小麦耐旱性的转基因过表达策略的实用性。
更新日期:2020-03-26
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