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Uranium tolerant phosphate solubilizing bacteria isolated from Gogi, a proposed uranium mining site in South India
Applied Geochemistry ( IF 3.4 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.apgeochem.2020.104523
S. Sowmya , P.D. Rekha , I. Yashodhara , N. Karunakara , A.B. Arun

Abstract Remediation of uranium contamination presents a significant environmental problem worldwide. Bioremediation has gained increasing importance as a feasible and eco-friendly strategy. Uranium tolerant phosphate solubilizing bacteria are considered as important candidates in the development of bioremediation technology. In this context, we have isolated bacteria from a proposed uranium mining site, Gogi in the Bhima river belt of Karnataka (South India) with special reference to phosphate solubilizers. Out of 270 bacteria isolated, 14 isolates solubilized 148.5–1226.6 mgL−1 phosphate from 5 g L−1 tri-calcium phosphate accompanied by drop in media pH from an initial 6.9 to pH values between 3.9 and 6.3. Phylogenetic analysis of 14 phosphate solubilizing bacteria by 16S rRNA gene sequencing grouped them into three phyla, namely Firmicutes, Proteobacteria and Actinobacteria. When tested for uranium sensitivity, 12 of the 14 phosphate solubilizing isolates showed significant (p

中文翻译:

从印度南部拟建的铀矿场 Gogi 中分离出的耐铀磷酸盐溶解细菌

摘要 铀污染的修复是世界范围内一个重大的环境问题。生物修复作为一种可行且环保的策略,其重要性日益增加。耐铀磷酸盐溶解细菌被认为是生物修复技术发展的重要候选者。在这种情况下,我们从卡纳塔克邦(南印度)比马河带的一个拟建铀矿场 Gogi 中分离出细菌,并特别提到了磷酸盐增溶剂。在分离出的 270 种细菌中,14 种分离物从 5 g L-1 磷酸三钙中溶解了 148.5–1226.6 mgL-1 磷酸盐,同时培养基 pH 值从最初的 6.9 下降到 3.9 和 6.3 之间的 pH 值。通过 16S rRNA 基因测序对 14 种溶磷细菌进行系统发育分析,将它们分为三个门,即厚壁菌门、变形菌和放线菌。当测试铀敏感性时,14 种磷酸盐溶解分离株中有 12 种表现出显着性(p
更新日期:2020-03-01
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