Abstract Due to a low ratio of chemical oxygen demand (COD) to total nitrogen (TN) and poor biodegradability of digested piggery wastewater, lack of a carbon source in subsequent biological treatment limits denitrification, resulting in an accumulation of NO2-N and NO3-N and a low denitrification efficiency. In this project, combined ultrasonication/alkali treatment of pig manure was used to generate a source of pig manure carbon source(PMC). This report mainly discusses the effects of adding the PMC on subsequent biological denitrification and microbial community changes. The experimental results showed that in subsequent biological treatment with the addition of 1.78% PMC, the effluent NO2-N and NO3-N were 0.02 mg·L−1 and 0.90 mg·L−1, respectively. The TN removal percentage was 72.29%, which was 62.29% higher than that obtained without an added carbon source and 36.74% higher than that obtained with added raw wastewater carbon (RWC). High-throughput sequencing revealed that the relative abundances of the dominant microflora Thauera and Thermomonas, which are related to denitrification, increased from 1.13% and 0.55% to 6.19% and 4.69%, respectively, after the addition of PMC. The addition of PMC improved the denitrification of digested piggery wastewater in subsequent biological treatment, and the denitrification effect was obvious and could enable waste resource utilization.

中文翻译：

---

猪粪作为传统养猪废水处理系统内碳源生物脱硝的效果

摘要 由于化学需氧量（COD）与总氮（TN）的比值低，消化后的猪场废水生物降解性差，在后续生物处理中缺乏碳源限制了反硝化，导致NO2-N和NO3- N和低反硝化效率。本项目采用超声/碱法联合处理猪粪，生成猪粪碳源（PMC）。本报告主要讨论添加PMC对后续生物反硝化和微生物群落变化的影响。实验结果表明，在后续生物处理中加入1.78%的PMC，出水NO2-N和NO3-N分别为0.02 mg·L-1和0.90 mg·L-1。TN去除率为72.29%，为62。比不添加碳源获得的高 29%，比添加原废水碳 (RWC) 获得的高 36.74%。高通量测序显示，加入PMC后，与反硝化作用相关的优势菌群Thauera和Thermomonas的相对丰度分别从1.13%和0.55%增加到6.19%和4.69%。PMC的加入提高了后续生物处理对消化后的猪场废水的反硝化效果，反硝化效果明显，可实现废弃物资源化利用。添加PMC后分别为55%至6.19%和4.69%。PMC的加入提高了后续生物处理对消化后的猪场废水的反硝化效果，反硝化效果明显，可实现废弃物资源化利用。添加PMC后分别为55%至6.19%和4.69%。PMC的加入提高了后续生物处理对消化后的猪场废水的反硝化效果，反硝化效果明显，可实现废弃物资源化利用。