Background

Polycystic ovary syndrome (PCOS), a common hormonal disorder in women, affects 4–18% of women of reproductive age worldwide. A higher prevalence of irritable bowel syndrome was found in women with PCOS. However, the effects and mechanism of PCOS on stomach and colon contractility remain unclear.

Aims

This study aims to evaluate the correlation between PCOS and gastrointestinal disorder.

Methods

Four-week-old female rats were subcutaneously implanted with pellets containing 7.5 mg of dihydrotestosterone for 13 weeks to create PCOS rat models. After vaginal smears, the estrus cycle stage was evaluated. Oral glucose tolerance test was performed after 90 days of treatment. All animals were killed at 17 weeks. The rats were fasted overnight and then anesthetized before decapitation, and the stomach fundus and colon were surgically removed and cultured in oxygenated Krebs solution. Acetylcholine and carbachol were used to evaluate the cholinergic system on contractility.

Results

The basal and stomach fundus responded with a reduced frequency and contractility in response to acetylcholine in the PCOS group. Moreover, no difference was found in the spontaneous stomach contractility induced by carbachol in both groups. Lower maximal colon muscle contractility was also found in response to acetylcholine stimulation in PCOS rats. Furthermore, lower maximal muscle contractility was found in response to extracellular calcium levels. MLC20 phosphorylation was also reduced in the gastrointestinal tissue in PCOS rats.

Conclusions

PCOS induces gastroparesis and reduces gastrointestinal muscle contractility. This effect is, at least partly, through reducing the responsiveness of acetylcholine and MLC20 phosphorylation.

中文翻译：

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多囊卵巢综合征大鼠模型中胃肠道收缩力的紊乱。

背景

多囊卵巢综合症（PCOS）是女性常见的荷尔蒙紊乱，影响了全世界4-18％的育龄妇女。在患有PCOS的女性中，肠易激综合征的患病率更高。但是，PCOS对胃和结肠收缩的作用和机制尚不清楚。

目的

本研究旨在评估PCOS与胃肠道疾病之间的相关性。

方法

将四周大的雌性大鼠皮下植入含有7.5 mg二氢睾丸激素的小丸，持续13周，以创建PCOS大鼠模型。阴道涂片后，评估发情周期阶段。治疗90天后进行口服葡萄糖耐量测试。在第17周处死所有动物。将大鼠禁食过夜，然后在断头之前将其麻醉，并通过外科手术切除胃底和结肠，并在含氧的克雷布斯溶液中培养。使用乙酰胆碱和卡巴胆碱评估胆碱能系统的收缩力。

结果

在PCOS组中，基底和胃底对乙酰胆碱的反应频率和收缩性降低。此外，两组中卡巴胆碱引起的自发性胃收缩能力均无差异。在对PCOS大鼠的乙酰胆碱刺激中，还发现较低的最大结肠肌肉收缩能力。此外，发现响应于细胞外钙水平较低的最大肌肉收缩力。在PCOS大鼠的胃肠道组织中MLC20磷酸化也降低了。

结论

PCOS引起胃轻瘫，并降低胃肠道肌肉收缩力。该作用至少部分地是通过降低乙酰胆碱和MLC20磷酸化的反应性来实现的。