OBJECTIVE To present a systematic review that provides updated information about proteins found in salivary fluid extracted strictly from ducts. METHODS The systematic review probing strategy was based on electronic databases word search (PubMed, EMBASE, LILACS, Web of Science, and Scopus). Risk of bias was assessed based on Joanna Briggs Institute Critical Appraisal Checklist for Studies Reporting Prevalence Data. RESULTS After 2 rounds of scrutiny, 12 articles were included, totaling 231 individuals (125 were healthy, 41 were elder individuals with radicular caries, 56 had primary Sjögren's syndrome, and 9 were patients who had received radiotherapy for head and neck cancer). The selected studies had no similarities among proteins found, demonstrating the need of standard reference in experimental methodology to obtain a thorough coverage of proteins. CONCLUSION Further studies are required to better determine the relative amount of proteins described in this study. It is essential to increase the number of samples, to perform similar collection techniques, to include other analyses methods such as mass spectrometry, and to perform the validation of some proteins using immunoassay techniques such as Elisa and Western blot. CLINICAL RELEVANCE Proteomic profile of saliva collected from ducts is essential to better understand the disease process, enabling the identification of biomarkers for specific clinical situations.

中文翻译：

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直接从导管收集的唾液的蛋白质组学特征：系统评价。

目的提供系统的综述，以提供有关严格从导管中提取的唾液中发现的蛋白质的最新信息。方法系统的评价探索策略基于电子数据库单词搜索（PubMed，EMBASE，LILACS，Web of Science和Scopus）。基于乔安娜·布里格斯研究所报告流行数据的研究的关键评估清单对偏倚风险进行了评估。结果经过2轮仔细检查，共纳入12篇文章，共231人（健康125例，老年患有根状龋的个体41例，患有原发性干燥综合征，其中9例接受了头颈癌放疗的患者）。所选的研究在发现的蛋白质之间没有相似之处，证明在实验方法中需要标准参考才能获得蛋白质的全面覆盖。结论需要进一步研究以更好地确定本研究中描述的蛋白质的相对量。必须增加样品数量，执行类似的收集技术，包括其他分析方法（例如质谱），并使用免疫测定技术（例如Elisa和Western blot）对某些蛋白质进行验证。临床相关性从导管收集的唾液的蛋白质组学特征对于更好地了解疾病过程至关重要，因此可以针对特定的临床情况识别生物标志物。必须增加样品数量，执行类似的收集技术，包括其他分析方法（例如质谱），并使用免疫测定技术（例如Elisa和Western blot）对某些蛋白质进行验证。临床相关性从导管收集的唾液的蛋白质组学特征对于更好地了解疾病过程至关重要，因此可以针对特定的临床情况识别生物标志物。必须增加样品数量，执行类似的收集技术，包括其他分析方法（例如质谱），并使用免疫测定技术（例如Elisa和Western blot）对某些蛋白质进行验证。临床相关性从导管收集的唾液的蛋白质组学特征对于更好地了解疾病过程至关重要，因此可以针对特定的临床情况识别生物标志物。