Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen.,Archives of Virology

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Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen.
Archives of Virology ( IF 2.7 ) Pub Date : 2019-12-23 , DOI: 10.1007/s00705-019-04495-4
Qi Zhou ₁ , Zhuangli Bi _{1,

2} , Dongdong Yin _{1,

3} , Xiangxue Gu ₁ , Zejun Xu ₁ , Rong Huang ₁ , Xue Xing ₁ , Kezong Qi ₃ , Guijun Wang _{1,

3}

Affiliation

Duck Tembusu virus (DTMUV) has caused significant economic losses in China since 2010. However, there is still a lack of effective methods to diagnose the disease caused by this virus, and especially to differentiate infection from vaccination. In this study, we established a novel indirect enzyme-linked immunosorbent assay (iELISA) and performed a retrospective serological survey for DTMUV in Anhui province, China. Our results show that the iELISA displayed high specificity sensitivity, and with no serological cross-reaction with other duck pathogens. These findings indicate that the newly developed iELISA could be a useful screening tool for large-scale monitoring of the epidemiology of DTMUV infection in ducks.

更新日期：2020-01-04

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