The aim of this work was the genetic typing of RVA isolates originating from pigs and human patients in Slovakia. Seventy-eight rectal swabs from domestic pigs and 30 stool samples from humans were collected. The whole VP7 (G genotypes), VP6 (I genotypes) and partial VP4 (P genotypes) ORFs were amplified by RT-PCR. Genetic variability was higher amongst porcine sequences, where four G genotypes (G3, G4, G5, G11), two P genotypes (P[6], P[13]) and one I5 genotype were detected. Human RVA strains were represented by two G genotypes (G1, G3), two I genotypes (I1, I2), and one P genotype (P[8]). Genetic analysis did not show a relationship between Slovakian porcine and human RVA strains, but phylogenetic grouping of some Slovakian porcine sequences with Hungarian human sequences in both G and P genotypes was observed.

中文翻译：

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猪和人类轮状病毒A分离株的斯洛伐克遗传变异。

这项工作的目的是对来自斯洛伐克的猪和人类患者的RVA分离株进行基因分型。收集了来自家猪的78个直肠拭子和来自人类的30个粪便样本。通过RT-PCR扩增了整个VP7（G基因型），VP6（I基因型）和部分VP4（P基因型）ORF。猪序列中的遗传变异性较高，其中检测到四种G基因型（G3，G4，G5，G11），两种P基因型（P [6]，P [13]）和一种I5基因型。人类RVA株由两个G基因型（G1，G3），两个I基因型（I1，I2）和一个P基因型（P [8]）代表。遗传分析未显示斯洛伐克猪和人RVA株之间的关系，但是观察到一些G和P基因型的斯洛伐克猪序列与匈牙利人序列的系统发育分组。