Soybean mosaic virus (SMV) infections can cause significant reduction in soybean yield and seed quality. Identifying SMV resistance genes in soybean and accordingly breeding resistant soybean varieties is an important approach to control soybean loss caused by SMV. Here, whole genome resequencing was performed on a pair of near‐isogenic line (NIL), one resistant (R) line and one susceptible (S) line to SC3, from Qihuang‐1 (resistant to SC3, R_SC3Q) × Nannong 1138‐2 (susceptible to SC3, r_SC3Q). Based on the distribution of single nucleotide polymorphisms (SNPs) and InDels in each chromosome, the R_SC3Q was mapped to a region between 27.4 and 29.8 Mbp in chromosome 13. Comparing the sequence and transcriptome data between R line and susceptible lines (S line and Willams82) to SC3, three R_SC3Q candidate genes (Glyma13g25950, Glyma13g25970 and Glyma13g26380) were identified. Silencing Glyma13g26380 in Qihuang‐1 increased the accumulation of SMV at 6 and 24 hpi. Subsequently, the SMV concentration in silenced plants decreased to the same level as that of the control. Thus, Glyma13g26380 is involved in the early resistance response of Qihuang No. 1 to SC3. These results provide a basis for elucidating the soybean resistance mechanism against SMV and breeding more resistant soybean varieties against SMV.

中文翻译：

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大豆抗大豆花叶病毒SC3候选基因的定位和功能分析

大豆花叶病毒（SMV）感染会导致大豆产量和种子质量大幅下降。鉴定大豆中的SMV抗性基因并因此育种抗性大豆品种是控制由SMV引起的大豆损失的重要方法。在这里，对全基因组重测序是在一对_Q3-1（抗SC3，R _SC3Q）×南农1138的近等基因系（NIL），一个抗性（R）和一个易感性（S）对SC3的系上进行的‐2（对SC3敏感，r _SC3Q）。基于每个染色体中单核苷酸多态性（SNP）和InDels的分布，R _SC3Q将其定位于染色体13中27.4和29.8Mbp之间的区域。比较R株系和易感株系（S株系和Willams82）与SC3之间的序列和转录组数据，鉴定了三个R _SC3Q候选基因（Glyma13g25950，Glyma13g25970和Glyma13g26380）。在qihuang -1中沉默Glyma13g26380可增加6和24 hpi的SMV积累。随后，沉默植物中的SMV浓度降至与对照相同的水平。因此，Glyma13g26380参与了奇黄1号对SC3的早期抗性反应。这些结果为阐明大豆对SMV的抗性机理和选育更具抗性的大豆品种提供了基础。