In Arabidopsis thaliana, AtMYB76 is an MYB transcription factor that has been shown to play multiple roles in the biosynthesis of aliphatic glucosinolates and fatty acids. However, in Brassica napus, the role of BnaA10.SOI.a, an AtMYB76 orthologue, in the accumulation of seed lipids remains unclear. In the present study, we obtained the BnaA10.SOI.a coding domain sequence from the Brassica napus cultivar 'Zhongshuang 11' and analysed its conserved protein domain. A subcellular localization experiment in tobacco leaf cells indicated that BnaA10.SOI.a functioned as a transcription factor. Our results demonstrated that the introduction of BnaA10.SOI.a into an Arabidopsis thaliana myb76‐2 mutant could restore wild‐type levels of seed fatty acid content. Moreover, the expression levels of six oil biosynthetic genes in developing seeds of myb76‐2 were restored to wild‐type levels by ectopic expression of BnaA10.SOI.a. These results improve our understanding of the function of BnaA10.SOI.a and provide a potential target for genetic manipulations of B. napus to improve oil quantity in seeds.

中文翻译：

---

BnaA10.SOI.a在油菜籽种子脂肪酸生物合成中的可能作用

在拟南芥中，AtMYB76是一种MYB转录因子，已显示在脂族芥子油苷和脂肪酸的生物合成中发挥多种作用。然而，在甘蓝型油菜，作用BnaA10.SOI.a，一个AtMYB76直系同源，在种子脂质的积累尚不清楚。在本研究中，我们从甘蓝型油菜品种“中双11”获得了BnaA10.SOI.a编码域序列，并对其保守的蛋白质结构域进行了分析。烟草叶细胞中的亚细胞定位实验表明，BnaA10.SOI.a充当转录因子。我们的结果表明，将BnaA10.SOI.a引入了拟南芥myb76-2突变体可以恢复种子脂肪酸含量的野生型水平。此外，通过异位表达BnaA10.SOI.a，myb76-2发育中种子中的六个油生物合成基因的表达水平恢复为野生型水平。这些结果提高了我们对BnaA10.SOI.a功能的理解，并为遗传改造油菜的种子提供了潜在的目标，以提高种子中的油量。