With an objective of developing the induced mutants for superior productivity and resistance to foliar diseases in peanut, two introgression lines (IL‐3 and IL‐4) derived from ABK genomes of peanut were subjected to gamma and sodium azide mutagenesis. Evaluation of M₁, M₂ and M₃ generations could identify foliar disease resistant and productive mutants. Large‐scale evaluation of M₄ mutants during the rainy season of 2017 confirmed the superiority of 12 mutants over the respective parents and the best check (GPBD 4). The selected mutants were resistant to leaf spots (early and late) and rust diseases, and significantly more productive than the parents and GPBD 4. ddRAD‐Sequencing of these mutants identified the single nucleotide polymorphisms (SNPs) when compared to their parents. The genes harbouring these structural mutations were also identified. However, no copy number variations (CNVs) were observed between the mutants and the parents. The selected mutants carried resistant‐type alleles at the marker loci linked to foliar disease resistance. These promising mutants (M₈) are currently under initial trials of variety development for commercial release.

中文翻译：

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花生（Arachis hypogaea）渗入系的抗叶病和高产突变体

为了开发诱导的突变体以提高花生的生产力和抗叶病性，对来自花生ABK基因组的两个渗入系（IL-3和IL-4）进行了伽玛和叠氮化钠诱变。对M ₁，M ₂和M ₃世代的评估可以确定抗叶病性和高产突变体。M ₄的大规模评估2017年雨季的突变体证实了12个突变体优于各自的亲本和最佳对照（GPBD 4）。选定的突变体对叶斑病（早期和晚期）和铁锈病具有抗性，并且比亲本和GPBD 4的生产力更高。与亲本相比，这些突变体的ddRAD测序确定了单核苷酸多态性（SNP）。还鉴定了具有这些结构突变的基因。但是，在突变体和亲本之间没有观察到拷贝数变异（CNV）。选定的突变体在与叶病抗性相关的标记位点携带抗性型等位基因。这些有前途的突变体（M ₈）目前正处于商业开发品种开发的初步试验中。