Seed fatty acid content is an important consideration for soybean produced for food, feed, and industrial applications. In this study, MCScanX was used to analyze the entire soybean genome to generate a collinearity block, which was then used to assess the collinearity among the soybean fatty acid quantitative trait loci (QTL) in the SoyBase database. The hub‐QTLs located in the Gm06, Gm07, and Gm10 segments were identified. The Kyoto Encyclopedia of Genes and Genomes and gene ontology databases were used to analyze the genes in hub‐QTL regions, resulting in the identification of 17 candidate genes related to soybean fatty acid content. Two lines with different fatty acid contents and a recurrent parent were selected from a chromosome segment substitution line library for a subsequent quantitative real‐time polymerase chain reaction (qRT‐PCR) assay to verify the candidate gene expression patterns. Four genes were related to the total soybean fatty acid content, while three genes were related to the content of specific fatty acid types. The results of this study may be relevant for the fine mapping of soybean fatty acid QTLs/genes.

中文翻译：

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基于大豆基因组共线性分析鉴定与脂肪酸含量相关的大豆基因

种子脂肪酸含量是用于食品，饲料和工业应用的大豆的重要考虑因素。在这项研究中，MCScanX用于分析整个大豆基因组以生成共线性块，然后将其用于评估SoyBase数据库中大豆脂肪酸定量特征基因座（QTL）之间的共线性。确定了位于Gm06，Gm07和Gm10网段的集线器QTL。使用《京都基因与基因组百科全书》和基因本体数据库对hub-QTL区域中的基因进行分析，从而鉴定出与大豆脂肪酸含量有关的17个候选基因。从染色体片段替代品系文库中选择了两个脂肪酸含量不同的品系和一个亲代亲本，用于随后的定量实时聚合酶链反应（qRT-PCR）分析以验证候选基因表达模式。四个基因与大豆总脂肪酸含量有关，而三个基因与特定脂肪酸类型的含量有关。这项研究的结果可能与大豆脂肪酸QTL /基因的精细定位有关。