Inheritance of fertility restorer gene in pigeonpea was studied using F₂ and BC₁F₁ populations derived from cross AL103A × IC245273. It was found to be controlled by single dominant gene. Out of 228 SSR primer pairs, 33 primer pairs showed parental polymorphism, while four primers were found polymorphic in bulk segregant analysis (BSA). These four primers viz., CcM 1615, CcM 0710, CcM 0765 and CcM 1522 were used for genotyping of F₂ population and were found to be placed at 3.1, 5.1, 28.1 and 45.8 cM, respectively. Two of them, CcM 1615 and CcM 0710, evinced clear and unambiguous bands for fertility restoration in F₂ population. The Rf gene was mapped on linkage group 6 between the SSR markers CcM 1615 and CcM 0710 with the distances of 3.1 and 5.1 cM, respectively. The accuracy of the CcM 1615 was validated in 18 restorers and six maintainer lines. The marker CcM 1615 amplified in majority of male restorer lines with a selection accuracy of 91.66%.

中文翻译：

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木豆A2杂种系统中生殖力恢复（Rf）基因的遗传和分子作图

利用源自杂交AL103A×IC245273的F ₂和BC ₁ F ₁种群研究了木豆育性恢复基因的遗传。发现它受单个显性基因控制。在228对SSR引物对中，有33对引物表现出亲本多态性，而在批量分离子分析（BSA）中发现4对引物具有多态性。使用这四个引物，即CcM 1615，CcM 0710，CcM 0765和CcM 1522进行F ₂群体的基因分型，发现分别位于3.1、5.1、28.1和45.8 cM。其中两个CcM 1615和CcM 0710为F _2的生育力恢复提供了清晰明确的条带人口。将Rf基因定位在SSR标记CcM 1615和CcM 0710之间的连接基团6上，其距离分别为3.1和5.1 cM。CcM 1615的准确性已在18个修复器和6个维护器系列中得到验证。标记CcM 1615在大多数雄性恢复系中扩增，选择精度为91.66％。