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Protein-coding changes preceded cis-regulatory gains in a newly evolved transcription circuit
Science ( IF 56.9 ) Pub Date : 2020-01-02 , DOI: 10.1126/science.aax5217
Candace S Britton 1, 2 , Trevor R Sorrells 1, 2 , Alexander D Johnson 1
Affiliation  

Generating a new transcriptional network Organismal novelties result from changes in transcriptional circuits. But what comes first, changes in regulatory protein or changes in cis-regulatory sequences? Britton et al. examine the Matα2 protein in a Saccharomycotina clade of fungi. They show that a newly evolved transcription circuit involving repression of the a-specific genes by the ancient homeodomain protein Matα2 occurred in two stages separated by millions of years. In the first stage, Matα2 acquired several coding changes followed by changes in cis-regulatory sequences. This clade-specific requirement explains how the coding changes of Matα2 were in place long before the new a-specific gene repression circuit arose. Science, this issue p. 96 A transcription circuit evolved by coding changes in an ancient regulator followed by regulatory changes in its target genes. Changes in both the coding sequence of transcriptional regulators and in the cis-regulatory sequences recognized by these regulators have been implicated in the evolution of transcriptional circuits. However, little is known about how they evolved in concert. We describe an evolutionary pathway in fungi where a new transcriptional circuit (a-specific gene repression by the homeodomain protein Matα2) evolved by coding changes in this ancient regulator, followed millions of years later by cis-regulatory sequence changes in the genes of its future regulon. By analyzing a group of species that has acquired the coding changes but not the cis-regulatory sites, we show that the coding changes became necessary for the regulator’s deeply conserved function, thereby poising the regulator to jump-start formation of the new circuit.

中文翻译:

在新进化的转录回路中,蛋白质编码变化先于顺式调节增益

生成新的转录网络 生物新奇源于转录回路的变化。但首先是调节蛋白的变化还是顺式调节序列的变化?布里顿等人。检查真菌的 Saccharomycotina 进化枝中的 Matα2 蛋白。他们表明,一个新进化的转录回路涉及古代同源域蛋白 Matα2 对 a 特异性基因的抑制,发生在相隔数百万年的两个阶段。在第一阶段,Matα2 获得了几个编码变化,随后是顺式调控序列的变化。这种特定于进化枝的要求解释了 Matα2 的编码变化是如何在新的 a 特异性基因抑制回路出现之前很久就存在的。科学,这个问题 p。96 一个转录回路是通过在一个古老的调节器中编码变化然后在其靶基因中发生调节变化而进化出来的。转录调节因子的编码序列和这些调节因子识别的顺式调节序列的变化都与转录回路的进化有关。然而,人们对它们如何协同进化知之甚少。我们描述了真菌的进化途径,其中一个新的转录回路(同源域蛋白 Matα2 的特异性基因抑制)通过这个古老调节器的编码变化进化,数百万年后,其未来基因的顺式调节序列发生变化调节器。通过分析一组获得编码变化但没有获得顺式调控位点的物种,
更新日期:2020-01-02
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