BACKGROUND Our preliminary bioinformatics analysis showed that lncRNA TINCR may absorb miR-214-5p by serving is sponge, while miR-214-5p targets ROCK1. This study aimed to investigate the interactions among these 3 factors in hepatocellular carcinoma (HCC). METHODS Expression of TINCR, ROCK1 and miR-214-5p in HCC and non-tumor tissues was detected by performing qPCR. The correlations among TINCR, ROCK1 and miR-214-5p in HCC tissues were analyzed by performing linear regression. Overexpression experiments were performed to analyze gene interactions. Cell proliferation was analyzed by CCK-8 assay. RESULTS We found that TINCR and ROCK1 were upregulated, while miR-214-5p was downregulated in HCC. TINCR and ROCK1 were positively correlated, while TINCR and miR-214-5p were not significantly correlated. In HCC cells, TINCR overexpression is followed by ROCK1 overexpression, while miR-214-5p overexpression induced the downregulation of ROCK1. In addition, TINCR and miR-214-5p did not affect the expression of each other. TINCR and ROCK1 overexpression led to increased rate of cancer cell proliferation, while miR-214-5p played an opposite role and reduced the effects of TINCR overexpression. Therefore, TINCR sponges miR-214-5p to upregulate ROCK1 in HCC, thereby promoting cancer cell invasion and migration.

中文翻译：

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lncRNA TINCR使miR-214-5p在肝细胞癌中上调ROCK1。

背景我们的初步生物信息学分析表明，lncRNA TINCR可以通过吸收海绵来吸收miR-214-5p，而miR-214-5p靶向ROCK1。这项研究旨在调查这3个因素在肝细胞癌（HCC）中的相互作用。方法通过qPCR检测TINCR，ROCK1和miR-214-5p在肝癌和非肿瘤组织中的表达。通过线性回归分析肝癌组织中TINCR，ROCK1和miR-214-5p之间的相关性。进行过表达实验以分析基因相互作用。通过CCK-8测定法分析细胞增殖。结果我们发现，在肝癌中，TINCR和ROCK1被上调，而miR-214-5p被下调。TINCR和ROCK1正相关，而TINCR和miR-214-5p无显着相关。在肝癌细胞中 TINCR过表达后是ROCK1过表达，而miR-214-5p过表达则诱导了ROCK1的下调。此外，TINCR和miR-214-5p互不影响表达。TINCR和ROCK1的过表达导致癌细胞增殖率增加，而miR-214-5p发挥相反的作用并降低TINCR的过表达。因此，TINCR海绵miR-214-5p在肝癌中上调ROCK1，从而促进癌细胞的侵袭和迁移。