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Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes.
Stem Cell Research & Therapy ( IF 7.5 ) Pub Date : 2020-01-03 , DOI: 10.1186/s13287-019-1527-z Lu Zhai 1 , Yuhua Liu 1 , Wenpiao Zhao 2 , Qingyun Chen 1 , Tao Guo 3 , Wei Wei 4 , Zhuchun Luo 1, 5 , Yanfeng Huang 1 , Cui Ma 1 , Feng Huang 3 , Xia Dai 1
Stem Cell Research & Therapy ( IF 7.5 ) Pub Date : 2020-01-03 , DOI: 10.1186/s13287-019-1527-z Lu Zhai 1 , Yuhua Liu 1 , Wenpiao Zhao 2 , Qingyun Chen 1 , Tao Guo 3 , Wei Wei 4 , Zhuchun Luo 1, 5 , Yanfeng Huang 1 , Cui Ma 1 , Feng Huang 3 , Xia Dai 1
Affiliation
BACKGROUND
To explore the effect of aerobic training (AT), resistance training (RT) or a combination of AT and RT (AT+RT) on the function of endothelial progenitor cells (EPCs) in mice with type 2 diabetes and the potential effective mechanisms METHODS: Eight-week-old db/db male mice were used as type 2 diabetic animal models in this study. Mice were randomly assigned to the control group (n = 5), AT group (n = 5), RT group (n = 5) and AT+RT group (n = 5). Mice in the control group remained sedentary with no specific training requirement. Mice were motivated to perform AT, RT or AT+RT by a gentle pat on their body for 3 or 4 days/week for 14 days. AT was performed by treadmill running, RT was performed by ladder climbing and AT+RT involved both AT and RT. Bone-derived EPCs were isolated after 14 days of the intervention. EPC expression of CD31, CD34, CD133, CD144 and VEGFR2 was detected by immunofluorescence staining. Fluorescence detection was performed on attached mononuclear cells to detect double-positive EPCs. We then explored the effect of caveolin-1 knockdown (lentiviral vector with caveolin-1-siRNA) on the proliferation and adherence of EPCs and the concentration of caveolin-1 and PI3K/AKT via western blot analyses.
RESULTS
Compared to the mice in the control group, the mice in the AT, RT and AT+RT groups presented significant increases in proliferation and adherence after 14 days of intervention. AT+RT induced an increase in EPC adherence, which was greater than that of the control, RT and AT groups. Caveolin-1 knockdown inhibited the EPC proliferative and adherent abilities. The AT+RT group showed higher levels of caveolin-1 and p-AKT than the control group, but these changes were decreased by caveolin-1-siRNA transfection.
CONCLUSION
Combined AT and RT is an effective way to improve EPC function through upregulation of caveolin-1 in mice with type 2 diabetes.
中文翻译:
有氧和阻力训练通过上调 2 型糖尿病小鼠的小窝蛋白-1 来增强内皮祖细胞功能。
背景 探讨有氧训练(AT)、阻力训练(RT)或AT+RT联合训练(AT+RT)对2型糖尿病小鼠内皮祖细胞(EPCs)功能的影响及可能的作用机制。方法:本研究以8周龄db/db雄性小鼠为2型糖尿病动物模型。将小鼠随机分配到对照组(n = 5)、AT 组(n = 5)、RT 组(n = 5)和 AT+RT 组(n = 5)。对照组的小鼠保持久坐不动,没有特定的训练要求。通过轻轻拍打它们的身体,小鼠被激励执行 AT、RT 或 AT+RT,每周 3 或 4 天,持续 14 天。AT通过跑步机进行,RT通过爬梯进行,AT+RT包括AT和RT。在干预 14 天后分离出骨源性 EPC。CD31的EPC表达,通过免疫荧光染色检测CD34、CD133、CD144和VEGFR2。对附着的单核细胞进行荧光检测以检测双阳性EPC。然后,我们通过蛋白质印迹分析探讨了caveolin-1 敲低(带有caveolin-1-siRNA 的慢病毒载体)对EPC 增殖和粘附以及caveolin-1 和PI3K/AKT 浓度的影响。结果与对照组小鼠相比,AT、RT和AT+RT组小鼠在干预14天后增殖和依从性显着增加。AT+RT 诱导 EPC 依从性增加,高于对照组、RT 组和 AT 组。Caveolin-1 敲低抑制了EPC 增殖和粘附能力。AT+RT组的caveolin-1和p-AKT水平高于对照组,但这些变化因caveolin-1-siRNA 转染而减少。结论 AT联合RT是通过上调2型糖尿病小鼠caveolin-1改善EPC功能的有效方法。
更新日期:2020-01-04
中文翻译:
有氧和阻力训练通过上调 2 型糖尿病小鼠的小窝蛋白-1 来增强内皮祖细胞功能。
背景 探讨有氧训练(AT)、阻力训练(RT)或AT+RT联合训练(AT+RT)对2型糖尿病小鼠内皮祖细胞(EPCs)功能的影响及可能的作用机制。方法:本研究以8周龄db/db雄性小鼠为2型糖尿病动物模型。将小鼠随机分配到对照组(n = 5)、AT 组(n = 5)、RT 组(n = 5)和 AT+RT 组(n = 5)。对照组的小鼠保持久坐不动,没有特定的训练要求。通过轻轻拍打它们的身体,小鼠被激励执行 AT、RT 或 AT+RT,每周 3 或 4 天,持续 14 天。AT通过跑步机进行,RT通过爬梯进行,AT+RT包括AT和RT。在干预 14 天后分离出骨源性 EPC。CD31的EPC表达,通过免疫荧光染色检测CD34、CD133、CD144和VEGFR2。对附着的单核细胞进行荧光检测以检测双阳性EPC。然后,我们通过蛋白质印迹分析探讨了caveolin-1 敲低(带有caveolin-1-siRNA 的慢病毒载体)对EPC 增殖和粘附以及caveolin-1 和PI3K/AKT 浓度的影响。结果与对照组小鼠相比,AT、RT和AT+RT组小鼠在干预14天后增殖和依从性显着增加。AT+RT 诱导 EPC 依从性增加,高于对照组、RT 组和 AT 组。Caveolin-1 敲低抑制了EPC 增殖和粘附能力。AT+RT组的caveolin-1和p-AKT水平高于对照组,但这些变化因caveolin-1-siRNA 转染而减少。结论 AT联合RT是通过上调2型糖尿病小鼠caveolin-1改善EPC功能的有效方法。